Jon J Amberg

Description of Work This spring (2014) we will measure Asian carp eDNA over time at a Missouri River site downstream of multiple spawning areas before and during spawning. We will measure the amount of Asian carp eDNA in water samples. The amount of eDNA will be related to the numbers of eggs and larvae counted in matched samples (water samples taken at the same time and place). The time since the eDNA was released by the carp will be estimated and these results will be related to the average age of AC eggs and larvae in matched samples. We will also test for substances which interfere with the eDNA measurement techniques and can lead to false negatives. We will also be validating markers developed by USGS and...

Categories: Project; Tags: Asian Carp, GLRI, Great Lakes Restoration Initiative, Great Lakes Restoration Initiative, Invasive Species, All tags... Template 66, Template 673, Fewer tags

Using dissolved carbon dioxide to alter the behavior of invasive Round Goby

Carbon dioxide (CO2) is under consideration as a chemical fish deterrent at pinch points in rivers (e.g., inside locks) to reduce unwanted fish passage. This study evaluated the behavioral responses of invasive round goby (Neogobius melanostomus) to dissolved CO2 in laboratory tanks. The goal of this project was to quantify CO2 concentrations that modify round goby behavior. More specifically, avoidance behavior (voluntary response) and loss of equilibrium (involuntary response) were quantified across 5, 15, and 25°C water temperatures. These data can be used to inform future field testing of CO2 as an invasive fish deterrent. Spreadsheets include data that were used to evaluate the efficacy of carbon dioxide (CO2)...

Categories: Data; Types: Citation; Tags: Round goby, USGS Science Data Catalog (SDC), Upper Midwest Environmental Sciences Center, avoidance, behavior, All tags... carbon dioxide, water temperature, Fewer tags

Carbon dioxide-induced mortality of four species of North American fishes

There is growing interest in the use of carbon dioxide (CO2) as a management tool for controlling invasive fishes. However, there is limited published data on susceptibility of many commonly encountered species to elevated CO2 concentrations. Our objective was to estimate the 24-h LC50 and LC95 of four fishes (Rainbow Trout Oncorhynchus mykiss, Common Carp Cyprinus Carpio, Channel Catfish Ictalurus punctatus, and Westslope Cutthroat Trout Oncorhynchus clarkii lewisi). In the laboratory, we exposed fingerlings to a range of levels of CO2 for 24-h in unpressurized, flow-through tanks. A Bayesian hierarchical model was developed to estimate the dose response relationship for each species of fish with associated uncertainty,...

Categories: Data; Tags: Bozeman Fish Technology Center; Bozeman, MT, Carbon Dioxide, LC95, USGS Science Data Catalog (SDC), biota, All tags... mortality, piscicide, Fewer tags

ANT Bait Common Carp Control Data

This is a dataset accompanying the 2017 publication “Assessing the efficacy of corn-based bait containing antimycin-a to control common carp populations using laboratory and pond experiments.” Data are included for the four experiments conducted described in this paper. First, data for a gavage experiment including mortality of carp per tank, fish metrics (lengths and weights), and water quality data (pH, dissolved oxygen, temperature). Second, data are included for the leaching trial including mortality of bluegill and carp, concentrations of antimycin in the tanks, fish metrics, and water quality metrics. Third, data are included for the species-specificity indoor trial. Included in this are mortality and sample...

Categories: Data; Tags: Cyprinis carpio, USGS Science Data Catalog (SDC), USGS Upper Midwest Environmental Sciences Center, La Crosse, Wisconsin, antimycin-a, common carp, All tags... invasive fish, management, toxins, Fewer tags

Bigheaded carp ichthyoplankton qPCR screening tool: data

This dataset includes information from ichthyoplankton sampling for silver carp and bighead carp collected from three main reaches in the Upper Mississippi River Basin, including the La Grange, Peoria, Starved Rock, and Marseilles reaches of the Illinois River, Pool 20 of Mississippi River, and the St. Croix River. Data include the number of bigheaded carp eggs and larvae identified using traditional manual sorting techniques, and genetic data derived from quantitative PCR (qPCR) and 4 bigheaded carp markers (SCTM4/5 and BHTM1/2) that were used to identify the amount of DNA in ichthyoplankton tow samples. These data provide information for natural resource agencies and managers who could use this qPCR screening...

Categories: Data; Tags: Hypophthalmichthys, Illinois, Illinois River, USA, Iowa, Midwest Region, USA, All tags... Minnesota, Mississippi River, USA, St Croix River, USA, USGS Science Data Catalog (SDC), Wisconsin, bighead carp, biota, eggs, genetic screening, larvae, silver carp, Fewer tags