Research Microbiologist
Email:
jhansen@usgs.gov
Office Phone:
206-526-6588
Fax:
206-526-6654
ORCID:
0000-0002-3006-2734
Location
6505 N.E. 65th Street
Seattle
, WA
98115
US
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Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Also included are water quality and chemical concentration data.
Tags: Aquatic Biology,
Columbia Environmental Research Center,
DNA sequencing,
Edwardsiella piscicida,
Environmental Health, All tags...
RNAseq,
USGS Science Data Catalog (SDC),
Western Fisheries Research Science Center laboratory,
alternative complement pathway analysis,
biota,
disease challenge,
estrogenic endocrine disrupting compounds,
histopathology,
immunomodulation,
largemouth bass,
liver,
polymerase chain reaction,
spleen,
survival,
transcriptomic changes, Fewer tags
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Several Francisella spp. including F. noatunensis are regarded as important emerging pathogens of wild and farmed fish. However, very few studies have investigated the virulence factors that allow these bacterial species to be pathogenic in fish. The Francisella Pathogenicity Island (FPI) is a well-described, gene-dense region encoding major virulence factors for the genus Francisella. PdpA is a member of the pathogenicity determining protein genes encoded by the FPI that are implicated in the ability of the mammalian pathogen, F. tularensis, to escape and replicate in infected host cells. Using a sacB suicide approach, we generated pdpA knockouts to address the role of PdpA as a virulence factor for F. noatunensis....
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Coho salmon have been shown to be highly sensitive to the tire transformation chemical 6PPD-quinone, with concentrations contained in stormwater sufficient to induce mortality in up to 90% of the entire fall coho run. Other salmonid species (chinook, sockeye, chum) are much less sensitive to 6PPD-quinone induced mortality. This data examines the varying species sensitivities across salmoids using in vivo exposures in juvenile fish. We then used established cell lines forom these species to assess their suitability for in vitro studies concerning species differences in toxicokinetics using fluorescence and absorbance-based plate assays.
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From 2018 to 2024 surface waters (fresh and brackish), groundwater, and stormwater (runoff, re-use) samples were collected from sites with varying landuse (agricultural, forested, and urban) and streamflow conditions and analyzed at the Organic Geochemistry Research Laboratory in Lawrence, Kansas for the tire antioxidant 6PPD (6-p-phenylenediamine; N-(1,3-Dimethylbutyl)-N’-phenyl-p-phenylenediamine) and ozonated transformation product 6PPD-quinone (6PPD-Q, 2-((4-Methylpentan-2-yl)amino)-5-(phenylamino)cyclohexa-2,5-diene-1,4-dione)). There were 78 urban sampling sites in Colorado (N=15), Connecticut (N=1), Georgia (N=1), Hawaii (N=22), Kansas (N=7), Michigan (N=1), Minnesota (N=1), North Carolina (N=1), Oklahoma...
Tags: 6PPD,
6PPD-Q,
Aquatic Biology,
Ecology,
Environmental Health, All tags...
Stormwater,
USGS Science Data Catalog (SDC),
United States,
Water Quality,
Water Resources,
environment,
inlandWaters,
liquid chromatography,
mass spectrometry,
stormwater,
tire,
tire and road wear particles,
urban,
water chemistry, Fewer tags
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6PPD-Quinone (6PPD-Q) was recently implicated in inducing mass pre-spawn mortality events in adult coho salmon (Oncorhynchus kisutch) in the Pacific Northwest. Since then, studies have reported highly variable toxicity among fish species, including within the salmonids. This dataset explores the sensitivity of coastal cutthroat trout (Oncorhynchus clarkii clarkii) at three different life stages: alevin, juveniles, and young of the year, and also includes analytically measured concentrations for the alevin and juvenile experiments.
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