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Raw MinION FASTQ datafiles corresponding to the paper “A comparison of avian influenza virus whole genome sequencing approaches using nanopore technology”

Raw sequencing data as generated by the five different methods used are provided for each of the three samples used in the comparison. The files are in FASTQ format as exported from the Oxford Nanopore’s MK1C using MinION flowcells. Files are labeled according to the method (as described in the paper) and the Sample ID). The MK1C exports data in blocks of 6000 reads per FASTQ file and all the FASTQ files from each method and sample are grouped in a common folder.

Categories: Data; Tags: CONUS, Emerging Infectious Disease, Highly Pathogenic Avian Influenza Virus, Methods Comparison, Methods Development, All tags... MinION, Nanopore sequencing, Next Generation Sequencing, Pathogen Discovery, USGS Science Data Catalog (SDC), Wildlife Disease, avian influenza, biota, Fewer tags

Epidemiologic and diagnostic data from two red foxes (Vulpes vulpes) and one fisher (Pekania pennanti) with highly pathogenic avian influenza H5N1 submitted to the USGS National Wildlife Health Center in 2022

This dataset includes epidemiology, clinical signs, gross and microscopic pathology, and virology data from two red foxes (Vulpes vulpes) and one fisher (Pekania pennanti) submitted to the USGS-National Wildlife Health Center for cause-of-death determination and confirmed positive for highly pathogenic avian influenza (HPAI) H5N1 by USDA’s National Veterinary Services Laboratories. The foxes were juveniles from North Dakota and the fisher was an adult from Wisconsin. Clinical signs included neurological deficits such as ataxia, lethargy, or paralysis. Gross and microscopic lesions included myocardial pallor, pulmonary and hepatic congestion, meningoencephalitis, interstitial pneumonia, myocardial necrosis, and hepatic...

Categories: Data; Tags: HPAI H5N1 2.3.4.4, Highly pathogenic avian influenza, USGS National Wildlife Health Center, Madison, Wisconsin, USGS Science Data Catalog (SDC), Wildlife Disease, All tags... biota, mammals, pathology, Fewer tags

Natural infections with Pigeon Paramyxovirus-1: Pathologic changes in Eurasian collared-doves (Streptopelia decaocto) and rock pigeons (Columba livia) in the USA: Data

We reviewed pathological findings and to a lesser extent epidemiological data from 70 free-ranging columbiforms naturally infected with Pigeon paramyxovirus-1 (PPMV-1) from 25 different PPMV-1 mortality events in columbiforms in the USA. In a subset of 17 birds from 10 of the studied outbreaks, we carried out immunohistochemistry targeting PPMV-1 nucleoprotein to determine the tissue distribution of the virus.

Categories: Data; Types: Citation; Tags: Arizona, Eurasian collared-dove, Kansas, Massachusetts, Mississippi, All tags... Montana, Nevada, New Mexico, Pennsylvania, Pigeon paramyxovirus serotype 1, Texas, USGS Science Data Catalog (SDC), Utah, avian paramyxovirus serotype 1, histopathology, immunohistochemistry, pathogenesis, rock pigeon, Fewer tags

Data set on reticuloendotheliosis in Hawaiian birds

Only one virus, Avipox, has been documented in wild birds in Hawaii. Here, using immunohistochemistry and PCR, we found that two native threatened Hawaiian geese, one with multicentric histiocytoma and another with toxoplasmosis and one Laysan albatross with avian pox were infected with reticuloendotheliosis virus (REV). The virus was isolated from one of the geese by cell culture. PCR surveys of other Hawaiian geese with various pathologies, avian pox cases, and pox viral isolates failed to reveal REV suggesting the virus is uncommon, at least in samples examined. The full genome of the Gag, Pol, and Env genes were sequenced for all three infected birds and revealed geographic divergence of the Pol gene suggesting...

Categories: Data; Tags: Hawaiian goose, Hawaiian islands, Laysan albatross, National Wildlife Health Center Honolulu Field Station, Pathology, All tags... USGS Science Data Catalog (SDC), Wildlife Disease, biota, immunohistochemistry, molecular virology, retrovirus, Fewer tags

Experimental infection of Mexican free-tailed bats (Tadarida brasiliensis) with SARS-CoV-2

We experimentally challenged wild Mexican free-tailed bats (TABR) with SARS-CoV-2 to determine the susceptibility, reservoir potential, and population impacts of infection in this species. Of nine bats oronasally inoculated with SARS-CoV-2, five became infected and orally excreted moderate amounts of virus for up to 18 days post inoculation. These five subjects all seroconverted and cleared the virus before the end of the study with no obvious clinical signs of disease. We additionally found no evidence of viral transmission to uninoculated subjects. These results indicate that while TABR are susceptible to SARS-CoV-2 infection, infection of wild populations of TABR would not likely cause mortality.

Categories: Data; Tags: Mexican free-tailed bats, SARS-CoV-2, USGS National Wildlife Health Center Madison, WI, USGS Science Data Catalog (SDC), Wildlife Disease, All tags... biota, Fewer tags