Daniel A Grear

Pathology and bacteriology of 178 tadpoles with histologically confirmed Severe Perkinsea Infections: Data

(i) This dataset presents macroscopic and microscopic pathological findings and bacteriology of 178 tadpoles diagnosed with Severe Perkinsea Infections. (ii) Specimen were collected and submitted to the NWHC as part of mortality investigations and collection of specimen from apparently healthy populations as part of ongoing amphibian health monitoring. (iii) Necropsies and gross evaluation of carcasses were carried out under a dissecting microscope. (iv) Severe Perkinsea Infection was confirmed in each specimen by histological observation of pathological changes in tissues with presence of Perkinsea-like organisms in at least one organ, including brain, eyes, gastrointestinal tract, gills, heart, liver, lungs, kidney,...

Categories: Data; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: USGS Science Data Catalog (SDC), Unites States of Ameica, amphibian, biota, case definition, All tags... emerging infectious disease, histopathy, pathogenesis, pathogenic Perkinsea Clade, ranavirus, tadpoles, Fewer tags

Population genetic analysis of the snake-infecting fungus, Ophidiomyces ophidiicola, in the eastern United States

Snake fungal disease (SFD; ophidiomycosis), caused by the pathogen Ophidiomyces ophidiicola (Oo), has been documented in wild snakes in North America and Eurasia, and is a potentially emerging disease in the eastern USA. However, a lack of historical disease data has made it challenging to determine whether Oo is a recent arrival to the USA or whether SFD emergence is due to other factors. Here, we examined the genomes of 82 Oo strains to determine the pathogen’s history in the eastern USA. This dataset contains metadata on the strains used in that study.

Categories: Data; Tags: Eastern United States, Fungal whole genome sequencing, Ophidiomyces, USGS Science Data Catalog (SDC), Wildlife Disease, All tags... biota, ophidiomycosis, snake fungal disease, Fewer tags

Evaluating regulations and surveillance as risk-mitigation to the emerging amphibian pathogen Bsal- Data release

Results of a literature search performed December 1, 2020. The tables in this document summarize the publications fitting in this search criteria and are split into the publications that update the list of international species that could carry Bsal (Appendix 1 Table S1). Number of individual live amphibians imported in categories of urodelan (salamander, newt, and related taxa) species restricted under the interim regulations and not listed, species not listed on the regulations that have had Bsal detected, notably Bombina spp., anuran species in the genus Rana, and Xenopus (including African clawed frogs) as a comparison of species not thought to be susceptible to Bsal, but commonly imported (Appendix 3 Table...

Categories: Data; Tags: Bombina, Bsal, Ecology, USGS Science Data Catalog (SDC), United States, All tags... Wildlife Disease, biota, chytridiomycosis, emerging infectious disease, risk, salamander, surveillance, wildlife trade, Fewer tags

Mercury concentrations in amphibian tissues across the United States, 2016-2021

Comma-separated values (.csv) file containing data related to amphibian sampling across the United States between 2016 and 2021. Data files contain mercury concentrations in amphibian and dragonfly tissues, mercury concentrations in sediment, as well as amphibian morphometrics, and habitat and climate characteristics where the samples were collected.

Categories: Data; Tags: Aquatic Biology, California, Colorado, Connecticut, Ecology, All tags... Environmental Health, Louisiana, Maine, Manitoba, Massachusetts, Montana, Oregon, Pennsylvania, USGS Science Data Catalog (SDC), Vermont, Virginia, Water Quality, Wildlife Biology, Wisconsin, Wyoming, biota, environment, inlandWaters, mercury contamination, Fewer tags

Data Release for Replicate amphibian swab and standard dilution of artificial DNA data to assess qPCR and sampling detection error for Batrachochytrium dendrobatidis.

We generated a data set from by collecting duplicate swabs from all amphibian individuals sampled at 5 sites (96 individuals) and analyzed these duplicate swabs running 3 subsamples of the extracted DNA in separate qPCR reactions. The resulting data set included 2 swabs per individual with 1 qPCR result and a 2nd swab with 3 qPCR results for detection of Batrachochytrium dendrobatidis (Bd) DNA. We generated data to assess the ideal laboratory functional relationship between quantity of target Bd DNA and detection, as well as limits of detection (LOD) and quantification (LOQ) for the assay, 5 µL of synthetic double-stranded Bd DNA template (gBlocks™ Gene Fragments, Integrated DNA Technologies, Coralville, IA) ranging...

Categories: Data; Tags: National Wildlife Health Center, Madison, WI, USGS Science Data Catalog (SDC), Wildlife Disease, biota