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The goal of this study was to develop a simple, cost effective method to mark fish through oral delivery of an indicator that could be qualitatively and quantitively assessed based on consumption. Our objectives were to 1) determine the concentration of orally administered Sudan Black B required for qualitative confirmation of ingestion, 2) trace the biodistribution of Sudan Black B in several fish tissues, and 3) quantify feed consumption by extracting orally delivered Sudan Black B from several tissues. Additionally, the time required to detect Sudan Black B in each tissue, effects between species, and optimal tissues for Sudan Black B extraction were determined. Methods were developed to quantify absorbance of...
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Locks and dams are possible management points to block the spread of invasive Asian carps in the United States. Infusion of carbon dioxide (CO2) into water is one deterrent strategy being considered at navigational structures to reduce upstream fish passage that would not directly interfere with lock and dam operations. The goal of this study was to determine lethal concentrations of CO2 to non-target species. Bluegill (Lepomis macrochirus) and Fathead minnows (Pimephales promelas) were exposed to CO2 continuously for 12 hours using a diluter system. Trials were performed on both species at target water temperatures of 5, 15, and 25°C. See related manuscript for additional details on experimental methods.
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