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Person

Barnett A Rattner

Research Physiologist
Eastern Ecological Science Center

Email: brattner@usgs.gov
Office Phone: 301-497-5671
Fax: 301-497-5744
ORCID: 0000-0003-3676-2843

Location
PTX - Gabrielson Office
12100 Beech Forest Road
Laurel , MD 20708
US

Supervisor: Alicia Berlin
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Reproductive Data for All Nests Monitored; Data for Mayfield Analysis; Eggshell thickness, nestling morphological measurements, nestling plasma stable isotopes, and nestling red blood cell oxidative DNA damage; Egg organochlorine pesticide, total PCB, flame retardants, and PCB toxic equivalent concentrations; Fish capture by osprey; Fish pools and size for contaminant analysis; Fish organochlorine pesticide, total PCB and flame retardants concentrations; Data for estimation of p,p’-DDE and total PCB biomagnification from fish to osprey eggs
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This dataset provides results for the following: (1) metals concentrations in soils surrounding breeding song bird nest sites; (2) metals concentrations in invertebrates (earthworm and grub composites) collected near nest sites; (3) metals concentrations and delta-aminolevulinic acid dehydratase (ALAD) activity in avian blood samples; and (4) metals concentrations, indicators of oxidative stress and DNA damage, and histopathology in liver and/or kidney tissues collected from breeding song birds.
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Brodifacoum toxicity in American kestrels (Falco sparverius) with evidence of increased hazard upon subsequent anticoagulant rodenticide exposure
A seminal question in ecotoxicology is the extent to which contaminant exposure evokes prolonged effects on physiological function and fitness. A series of studies were undertaken with American kestrels ingesting environmentally realistic concentrations of the second-generation anticoagulant rodenticide (SGAR) brodifacoum (BROD). Kestrels fed BROD at 0.3, 1.0 or 3.0 µg/g diet wet wt for 7 d exhibited dose-dependent hemorrhage, histopathological lesions and coagulopathy (prolonged prothrombin and Russell’s viper venom times). Following termination of a 7 d exposure to 0.5 µg BROD/g diet, prolonged blood clotting time returned to baseline values within a week, but BROD residues in liver and kidney (terminal half-life...
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A heterologous thrombin clotting time assay (TCT) was used to measure the time for conversion of fibrinogen to fibrin using commercially available reagents. Human reference material included in the kit was diluted with imidazole buffered saline (IBS; 0.0125M imidazole-0.109 M sodium chloride, pH7.4) to generate a standard curve. Each kestrel plasma sample was thawed at 37ºC and diluted with IBS, and following incubation at 37ºC, the reaction was then initiated by the addition of bovine thrombin reagent supplied with the assay kit, with clotting time measured to 0.1 s. Fibrinogen concentration was determined in a single assay for each of the 3 study trials, with reference samples interspersed among study samples....
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Range finding trial in which kestrels were fed diets containing varying quantities of brodifacoum and signs of intoxication were monitored.
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