Tonie E Rocke

SPV field trials bait uptake data

These data were collected as part of a field trial to test the effectiveness of a sylvatic plague vaccine (see Rocke et al., 2017 for details). Vaccine and control plots were selected randomly from the available pairs at each location. Baits containing Rhodamine B, a biomarker, were distributed at each plot. At least 1 week and no more than 2 months post-baiting each year, local collaborators captured, marked, and sampled prairie dogs. Hair and whisker samples were collected from up to 50 unique prairie dogs from each plot each year. Sex, age, weight, and the identity of all current-year and prior-year recaptures were recorded for each captured animal. In the laboratory, hair/whiskers were assessed for the presence...

Categories: Data; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Arizona, Awapa Plateau, Buffalo Gap National Grasslands, Cedar City, Charles M. Russell National Wildlife Refuge, All tags... Colorado, Coyote Basin, Espee Ranch, Gunnison, Larimer, Lower Brule, Montana, Pitchfork Ranch, Rita Blanca National Grasslands, South Dakota, Texas, USGS Science Data Catalog (SDC), Utah, Wind Cave National Park, biota, health, prairie dogs, sylvatic plague vaccine, Fewer tags

In vitro expression, immunogenicity, and efficacy data from recombinant raccoon poxvirus-vectored rabies vaccine candidates tested in mice

This work is part of an experimental trial to develop and assess novel recombinant raccoonpox virus (RCN) rabies vaccines in the mouse model, for potential use in bats. Briefly, our research group previously developed a recombinant RCN vaccine candidate expressing a mosaic glycoprotein (MoG) gene that protected mice and big brown bats when challenged with rabies virus (RABV). We developed two new recombinant RCN candidates expressing MoG (RCN-tPA-MoG and RCN-SS-TD-MoG) with the aim of improving RCN-MoG. We assessed and compared in vitro expression, in vivo immunogenicity, and protective efficacy in vaccinated mice challenged intracerebrally with RABV. In this data set, we share results of immunofluorescence assay...

Categories: Data; Tags: A/J mice, National Wildlife Health Center, Madison, WI, RABV, USGS Science Data Catalog (SDC), University of Wisconsin-Madison, Madison, WI - Hanson Biomedical Sciences, All tags... Wildlife Disease, biota, rabies, rabies virus, raccoon poxvirus, recombinant, vaccine, Fewer tags

Serology, survival, and detection of rabies data from vaccination trials in the common vampire bat (Desmodus rotundus) using a recombinant raccoon poxvirus-vectored mosaic rabies vaccine candidate

This data set provides biological information from of a captive colony of common vampire bats (wild-caught and born in captivity) that were part of an experimental study on vaccination. It includes results obtained from different assays: RT-PCR, serum neutralization, Rabies direct-fluorescent antibody test, performed periodically during the study (Sep 2018 – May 2020).

Categories: Data; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Rabies, San Luis Potosi, Mexico, Serology, USGS National Wildlife health Center, Madison, WI, USA, USGS Science Data Catalog (SDC), All tags... Wildlife Disease, biota, shedding, survival, vaccination, vaccine treatment, vampire bats, wildlife disease, Fewer tags

Impact of the oral Sylvatic Plague Vaccine on Non-target Small Rodents: Data

In 2013, a large blinded, paired placebo-controlled field trial for the prairie dog oral sylvatic plague vaccine started in the Western US. On 17 paired plots, vaccine and placebo plots, small rodents were trapped annually for 3-5 consecutive nights (when weather allowed). Up on capture, we documented the trap numbers and the processed animals. We noted standard biological information (species, sex, age) and took samples (fleas, blood, hair and whiskers). When logistics allowed we also trapped diurnal animals. Hair and whisker samples were taped to a transparent sheet and scored for the presence (high dose 1, low dose 2) or absence (0) of Rhodamine B fluorescence (RB dataset)

Categories: Data; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Awapa Plateau, Bend, Buffalo Gap National Grasslands and Wind Cave National Park, Charles M. Russell National Wildlife Refuge, Meeteetse East, All tags... Meeteetse West, Montana, Oral Sylvatic Plague Vaccine (SPV), Parowan area, Pitchfork Ranch, South Dakota, USGS Science Data Catalog (SDC), Utah, Vernal area, Wyoming, biota, health, Fewer tags

Plague positive mouse fleas on mice prior to plague outbreaks in black-tailed and white-tailed prairie dogs

In 2013, a large blinded, paired placebo-controlled field trial for the prairie dog oral sylvatic plague vaccine started in the Western US. On 17 paired plots, vaccine and placebo plots, small rodents were trapped annually for 3-5 consecutive nights (when weather allowed) and high elevation Utah plots where plague was active were more frequently trapped in 2014 and 2015. In the dataset the prevalence of flea infestation was recorded for the first annual summer sampling, it was summarized for all small rodent species caught and deer mice (Peromyscus maniculatus). Infestations were either for all flea species collected or for Aetheca wagneri only (our most abundant flea species). We used this data to assess if plague...

Categories: Data; Tags: Montana, Northern grasshopper mice, South Dakota, USGS Science Data Catalog (SDC), Utah, All tags... Wildlife Disease, Wyoming, Yersinia pestis, biota, deer mice, fleas, mice, plague, Fewer tags