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This data release includes all of the data presented in the peer-reviewed publication “Life at the frozen limit: Microbial Carbon Metabolism Across a Late Pleistocene Permafrost Chronosequence”. We collected permafrost from a Pleistocene chronosequence (19 ka to 33 ka) to examine (1) changes in the functional genetic potential of extant microbial communities to metabolize polysaccharides, (2) shifts in the quantity and quality of anions and dissolved nitrogen, and (3) changes in the molecular composition of dissolved organic matter. The data released herein shows that the age of permafrost had a marked effect on both the molecular composition of dissolved OC and the microbial community. Here we clearly demonstrate...
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Abstract (from Microbiome): Background Winter carbon loss in northern ecosystems is estimated to be greater than the average growing season carbon uptake and is primarily driven by microbial decomposers. Viruses modulate microbial carbon cycling via induced mortality and metabolic controls, but it is unknown whether viruses are active under winter conditions (anoxic and sub-freezing temperatures). Results We used stable isotope probing (SIP) targeted metagenomics to reveal the genomic potential of active soil microbial populations under simulated winter conditions, with an emphasis on viruses and virus-host dynamics. Arctic peat soils from the Bonanza Creek Long-Term Ecological Research site in Alaska were incubated...
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