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Growing season CH4 fluxes were monitored over a two year period following the start of ecosystem-scale manipulations of water table position and surface soil temperatures in a moderate rich fen in interior Alaska. The largest CH4 fluxes occurred in plots that received both flooding (raised water table position) and soil warming, while the lowest fluxes occurred in unwarmed plots in the lowered water table treatment. A combination of treatment and soil hydroclimate variables explained more than 70% of the variation in ln-transformed CH4 fluxes, with mean daily water table position representing the strongest predictor. We used quantitative PCR of the -subunit of mcr operon to explore the influence of soil climate...
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