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Stephen Spear

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This dataset contains raw sequence data collected from an eDNA metabarcoding project to detect freshwater mussel species across two sites in Georgia (Spring Creek and Flint River) and one drainage in Missouri (Big Piney River). The eDNA samples were collected from each stream using dead-end ultra filtration (D-HFUF) with eDNA extracted from filters. We used two previously published primer sets designed to amplify freshwater mussels, one that amplified the mitochondrial cytochrome c oxidase I (COI) region and one that amplified the NADH dehydrogenase subunit I region, with negative control and mock community samples included. Samples were individually indexed, pooled, and sequenced on an Illumina MiSeq high-throughput...
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Determining Priority Amphibian and Reptile Conservation Areas in the South Atlantic landscape, and assessing their efficacy for cross-taxa conservation
The proposed project focuses upon two major goals:1. Designate Priority Amphibian and Reptile Conservation Areas (PARCAs) in the South Atlantic Landscape, and develop an adaptive management plan for those areas.2. Determine whether PARCAs provide an effective strategy for integrated, long-term conservation of biodiversity and cultural resources in the South Atlantic Landscape; and develop a comprehensive biodiversity protection plan for the SALCC.To achieve these goals, we have identified the following objectives:Objective 1. Identify areas within the SALCC that are vitally important to preserving the rich diversity of amphibians and reptiles in the region, focusing upon rare species distribution, diversity, and...
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