Integrating data into a spatial framework After acquiring data, variables were attributed to a national stream coverage for use in assessment following Wang et al. (2011). The National Hydrography Dataset Version 1 (NHDV1) is a 1:100,000 scale representation of streams from throughout the conterminous United States. The NHDV1 identifies stream reaches as sections of streams occurring between confluences (Figure 2). We attributed all data to stream reaches (i.e., fish data, fragmentation metrics by dams) or to local catchments and 90m buffers draining to stream reaches (i.e., human land uses, mining activities, impervious surfaces, etc.). Local catchments (watersheds) and buffers are the land areas draining directly to a stream reach. [...]