Skip to main content

Ethanol and sodium acetate as a preservation method to delay degradation of environmental DNA: Data

Dates

Publication Date
Start Date
2014-03-04
End Date
2014-03-12

Citation

Ladell, B.A., Walleser, L.R., McCalla, G., Erickson, R.A., and Amberg, J.J., 2018, Ethanol and sodium acetate as a preservation method to delay degradation of environmental DNA: Data: U.S. Geological Survey data release, https://doi.org/10.5066/F7FB527Z.

Summary

This data contains environmental DNA (eDNA) sample assay results that were collected from water samples taken from a tank housing Asian carp and placed either on ice or at room temperature. At both treatment temperatures, water samples were left untreated or were treated with an ethanol and sodium acetate solution (EtOH-NaAc). This was done to evaluate an ethanol and sodium acetate solution to maintain the integrity of the DNA samples for the time between collection and lab testing. Every day for 6 days following collection, a subset of the samples was removed from each treatment and DNA was extracted and nuclear and mitochondrial markers were assayed with qPCR. Results showed comparable persistence of DNA between untreated iced samples [...]

Contacts

Attached Files

Click on title to download individual files attached to this item.

Ethanol_and_sodium_acetate_as_a_preservation_method_to_delay_degradation_of_environmental_DNA.xml
Original FGDC Metadata

View
25.18 KB application/fgdc+xml
Mitochon_Marker.csv 3.41 KB text/csv
Nuclear_Marker.csv 3.44 KB text/csv

Purpose

The data were collected to determine if environmental DNA could still be detected by PCR after being stored in an ethanol/sodium acetate solution rather than the conventional storage on ice.

Communities

  • Upper Midwest Environmental Sciences Center (UMESC)

Tags

Provenance

Data source
Input directly

Additional Information

Identifiers

Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/F7FB527Z

Item Actions

View Item as ...

Save Item as ...

View Item...