The effects of 28-day exposure to elevated carbon dioxide on survival, growth and condition of juvenile life stage of Lampsilis siliquoidea and Lampsilis higginsii mussels: Data

Dates

Publication Date: 2018-04-25
Start Date: 2015-04-01
End Date: 2016-04-30

Citation

Waller, D.L. and Jackson, C.A., 2018, The effects of 28-day exposure to elevated carbon dioxide on survival, growth and condition of juvenile life stage of Lampsilis siliquoidea and Lampsilis higginsii mussels: Data: U.S. Geological Survey data release, https://doi.org/10.5066/F78W3CMT.

Summary

The use of carbon dioxide to control aquatic invasive species (AIS) prompted an evaluation of its effects on native freshwater mussels that may reside in areas of infusion. We exposed juvenile mussels (~6-mo old) of two species, the fat mucket (Lampsilis siliquoidea) and the federally endangered Higgins’ Eye (L. higginsii) to CO2 concentrations proposed for AIS control (24,000 – 96,000 µatm pCO2 ) for 28 d followed by a 14-d recovery period and compared their survival, growth, and behavior. Additionally, tissue and shell condition and transcriptomic response of four genes were measured in L. siliquoidea. Survival was similar between species; the 14-d postexposure LC50 for L.higginsii was 61,000 µatm pCO2 (95% CL 46,000-87,700 µatm) [...]

Summary

The use of carbon dioxide to control aquatic invasive species (AIS) prompted an evaluation of its effects on native freshwater mussels that may reside in areas of infusion. We exposed juvenile mussels (~6-mo old) of two species, the fat mucket (Lampsilis siliquoidea) and the federally endangered Higgins’ Eye (L. higginsii) to CO2 concentrations proposed for AIS control (24,000 – 96,000 µatm pCO2 ) for 28 d followed by a 14-d recovery period and compared their survival, growth, and behavior. Additionally, tissue and shell condition and transcriptomic response of four genes were measured in L. siliquoidea. Survival was similar between species; the 14-d postexposure LC50 for L.higginsii was 61,000 µatm pCO2 (95% CL 46,000-87,700 µatm) and for L. siliquoidea was 69,100 µatm pCO2 (95% CL 64,000-75,100 µatm). Mean shell growth during exposure of both species was reduced in CO2 treatments with the exception of the L. higginsii group in the lowest concentration. Mean growth of both species was also reduced during the recovery period in treatments ≥61,000 µatm pCO2 while growth was comparable among mussels in the control and lower treatment groups. Both tissue (dry tissue weight/shell length) and shell condition (dry shell weight/shell length) were reduced in CO2 treatment groups indicating that somatic tissue growth and shell mass were adversely affected by hypercapnia. Carbon dioxide exposure increased the number of mussels that were unburied and that exhibited behavior indicative of narcotization. Recovery from narcotization occurred within 2 d in L. siliquoidea, whereas 33% of L. higginsii in 61,000 µatm pCO2 remained unburied at 4 d. Of the four genes that we targeted for qPCR, the most significant treatment related change was seen in chitinase synathase (CHS) expression. CHS mRNA exhibited a significant ~3 fold increase in the 61,000 µatm pCO2 treatment relative to the control and ~2 increase relative to the two lowest pCO2 treatments at 28 d. We conclude that elevated CO2 concentrations in freshwater pose a risk to juvenile unionid mussels at the concentrations proposed for AIS control. However, the effects of CO2 on native mussels will depend on the application mode (continuous, pulsed) and target concentration that is deployed. Lampsilis siliquoidea was a reasonable surrogate for L. higginsii with regard to survival, but may underestimate sublethal effects (growth, behavior-related mortality) of CO2 on the endangered species.

Contacts

Point of Contact :: Diane L Waller
Originator :: Diane L Waller, Craig A Jackson
Metadata Contact :: Diane L Waller
Distributor :: U.S. Geological Survey - ScienceBase
SDC Data Owner :: Upper Midwest Environmental Sciences Center
USGS Mission Area :: Ecosystems

Attached Files

Click on title to download individual files attached to this item.

Ammonia data.csv		2.39 KB	text/csv
ATPase exposure.csv		3.11 KB	text/csv
ATPase post_exposure.csv		4.15 KB	text/csv
behavior 4_1pe.csv		4 KB	text/csv
behavior d1_12.csv		13.86 KB	text/csv
camoudlin exposure.csv		3.24 KB	text/csv
camoudlin post_exposure.csv		4.34 KB	text/csv
chitin exposure.csv		3.06 KB	text/csv
chitin post_exposure.csv		4.1 KB	text/csv
CO2 data three measures for SAS.csv		13.58 KB	text/csv
defesin exposure.csv		4.28 KB	text/csv
defesin post_exposure.csv		5.32 KB	text/csv
Effects of 28-day exposure to elevated carbon dioxide on survival, growth and condition of juvenile life stage of Lampsilis siliquoidea and Lampsilis higginsii mussels.xml Original FGDC Metadata	View	104.94 KB	application/fgdc+xml
Mussel dry tissue and shell data for sas.csv		2.17 KB	text/csv
Mussel measurement data for sas.csv		26.31 KB	text/csv
Mussel mortality for SAS.csv		1.3 KB	text/csv
Water Chem data.csv		1.06 KB	text/csv
water quality for sas.csv		21.04 KB	text/csv

Related External Resources

Type: Publication that references this resource

Lethal and sublethal responses of native mussels (Unionidae: Lampsilis siliquoidea and L. higginsii) to elevated carbon dioxide	https://doi.org/10.1139/cjfas-2017-0543

Purpose

The data summarize the effects of carbon dioxide infusion for 28-d on the survival, shell growth, condition, genomic expression, and behavior of juvenile freshwater mussels. The project will be used to determine the risk to native mussels of using carbon dioxide as a tool to control Asian carp movements.

The effects of 28-day exposure to elevated carbon dioxide on survival, growth and condition of juvenile life stage of Lampsilis siliquoidea and Lampsilis higginsii mussels: Data

Dates

Citation

Summary

Summary

Contacts

Attached Files

Purpose

Communities

Tags

Provenance

Additional Information

Identifiers

Item Actions

View Item as ...

Save Item as ...

View Item...