Hawaii Island Environmental Sampler Comparison 2016-2018
Dates
Publication Date
2019-02-26
Start Date
2016-07-12
End Date
2018-04-01
Citation
Roy, K.A., Atkinson, C.T., and Granthon, C., 2019, Hawaii Island Environmental Sampler Comparison 2016-2018: U.S. Geological Survey data release, https://doi.org/10.5066/P9QN1HBT.
Summary
These data include metadata and associated data files associated with the manuscript, "Economical Environmental Sampler Designs for Detecting Airborne Spread of Fungi Responsible for Rapid ʽŌhiʽa Death." These data include a total of 8 datasets used for both controlled and field studies evaluating the use of Active (with battery operated fan) and Passive (dependent on wind) USGS Environmental Samplers on Hawaii Island between 2016-2018. Samplers were operated under controlled laboratory and field conditions with a commercial sampler (Rotorod® Model 20) to compare efficacy in capturing synthetic polyethylene spheres (12 - 160 µm in diameter) and also Xyleborus spp. boring dust (frass) known to contain the fungi responsible for Rapid [...]
Summary
These data include metadata and associated data files associated with the manuscript, "Economical Environmental Sampler Designs for Detecting Airborne Spread of Fungi Responsible for Rapid ʽŌhiʽa Death." These data include a total of 8 datasets used for both controlled and field studies evaluating the use of Active (with battery operated fan) and Passive (dependent on wind) USGS Environmental Samplers on Hawaii Island between 2016-2018. Samplers were operated under controlled laboratory and field conditions with a commercial sampler (Rotorod® Model 20) to compare efficacy in capturing synthetic polyethylene spheres (12 - 160 µm in diameter) and also Xyleborus spp. boring dust (frass) known to contain the fungi responsible for Rapid ʽŌhiʽa Death (Ceratocystis lukuohia and C. huliohia). Samplers were also tested at a field site with confirmed ROD mortality to demonstrate their ability to detect Ceratocystis DNA in beetle frass from infected trees and wood chips associated with tree felling under a range of environmental conditions. Data files consists of particle counts, particle measurements, and results of qPCR (quantitative polymerase chain reaction) tests to determine presence or absence of Ceratocystis DNA on tape strips that were exposed in samplers and tape strips that were spiked with known numbers of Ceratocystis spores to determine sensitivity of the methodology.
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Hawaii Island Environmental Sampler Comparison 2016-2018 Landing Page.xml Original FGDC Metadata
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Crop_Cage_Experiment.jpg “Dr. Carter Atkinson using various environmental samplers. Photo by Kylle Roy. ”
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Related External Resources
Type: Related Primary Publication
Atkinson, C.T., Roy, K., Granthon, C. 2019. Economical Environmental Sampler Designs for Detecting Airborne Spread of Fungi Responsible for Rapid `Ōhi`a Death. Hilo, Hawaii: University of Hawaii at Hilo Hawaii Cooperative Studies Unit, Technical Report HCSU-087. 37 pages. Available at: http://hdl.handle.net/10790/4568
These data were collected to determine efficacy of two new environmental samplers for detecting airborne spread of the fungi responsible for Rapid ʽŌhiʽa Death. The new designs are more cost effective than commercial devices that have been used in the agricultural and health sciences industry. Their low costs makes it feasible to deploy large numbers of samplers across large landscapes for monitoring spread of the disease.
Preview Image
Dr. Carter Atkinson using various environmental samplers. Photo by Kylle Roy.