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  1. Assessing cell line models for species differences in 6PPD-quinone sensitivity

Assessing cell line models for species differences in 6PPD-quinone sensitivity

Dates

Publication Date
Start Date
2021-12-02
End Date
2022-10-07

Citation

Greer, J.B., Dalsky, E.M., Lane, R.F., and Hansen, J.D., 2023, Assessing cell line models for species differences in 6PPD-quinone sensitivity: U.S. Geological Survey data release, https://doi.org/10.5066/P98HOI3I.

Summary

Coho salmon have been shown to be highly sensitive to the tire transformation chemical 6PPD-quinone, with concentrations contained in stormwater sufficient to induce mortality in up to 90% of the entire fall coho run. Other salmonid species (chinook, sockeye, chum) are much less sensitive to 6PPD-quinone induced mortality. This data examines the varying species sensitivities across salmoids using in vivo exposures in juvenile fish. We then used established cell lines forom these species to assess their suitability for in vitro studies concerning species differences in toxicokinetics using fluorescence and absorbance-based plate assays.

Contacts

Point of Contact :
Justin B Greer
Originator :
Justin B Greer, Ellie M. Dalsky, Rachael F Lane, John D Hansen
Metadata Contact :
Justin B Greer
Distributor :
U.S. Geological Survey - ScienceBase
Publisher :
U.S. Geological Survey
SDC Data Owner :
Western Fisheries Research Center
USGS Mission Area :
Ecosystems

Attached Files

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final_metadata.xml
“Metadata describing data collected to evaluate in vivo mortality and cell line t”
Original FGDC Metadata
View 		30.61 KB application/fgdc+xml
6ppd_cell_line_data.xls
“In vivo mortality and cell line toxicity measurements” 		432 KB application/vnd.ms-excel

Purpose

These data were collected to provide cell line based platforms for assessing stormwater toxicity across salmonids with in vitro assays in order to reduce the use of whole animal studies. The data should be used to confirm the viability of cellular assays as a viable alterative to whole-animal studies for 6PDD-quinone and related compounds.

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Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P98HOI3I

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