Carbon sequestration has been well recognized as a viable option to slow the rise in atmospheric greenhouse gas concentration. The main goals of this study were to assess the carbon sequestration potential (CSP) by afforestation of marginal agricultural land (MagLand) and to identify hotspots for potential afforestation activities in the U.S. Midwest region (Michigan (MI), Indiana (IN), Ohio, Kentucky (KY), West Virginia, Pennsylvania (PA) and Maryland (MD)). The 1992 USGS National Land Cover Dataset and the State Soil Geographic (STATSGO) database were used to determine MagLand. Two forest types (coniferous and deciduous) and two management practices (short-rotation versus permanent forest) were combined to form four afforestation [...]