A five-year record of streamflow and chemical sampling data was evaluated to assess the effects of large-scale prairie restoration on transport of NO3–N, Cl, and SO4 loads from paired 5000-ha watersheds located in Jasper County, Iowa. Water quality conditions monitored during land use conversion from row crop agriculture to native prairie in the Walnut Creek watershed were compared with a highly agricultural control watershed (Squaw Creek). Combining hydrograph separation with a load estimation program, baseflow and stormflow loads of NO3–N, Cl, and SO4 were estimated at upstream and downstream sites on Walnut Creek and a downstream site on Squaw Creek. Chemical export in both watersheds was found to occur primarily with baseflow, [...]