The goal of this study is to use eDNA as a cost effective tool for documenting the occurrence and distribution of ESA-listed spring-chinook (Oncorhynchus tshawytscha) throughout the Okanogan and Methow watersheds in an effort to map habitat use and connectivity. Our approach will use eDNA to detect low density spring-chinook in filtered water samples to inventory distribution and re-colonization of tributaries at strategically-sampled points throughout entire stream networks. This approach will provide one of the first applications of eDNA technology for monitoring distribution of native fish species of conservation concern. The findings will help the Colville Tribe Fisheries Managers assess aquatic connectivity for chinook salmon and provide baseline data for future evaluations of anthropogenic stressors, such as climate change, land use, and invasive species.
FY2012Objectives:
Aid resource managers in focused resource conservation by developing & publishing genetic primer/probe combinations along with protocols to detect chinook salmon infiltered water samples using eDNA.Track management efforts by mapping the current distribution of ESA-listed spring-chinook throughout the Methow and Okanogan watersheds.Provide baseline data for documenting success of spring-chinook reintroduction into the Okanogan watershed. These data will also allow managers to better understand processes of connectivity and colonization in the Okanogan watershed.Work with state, federal and tribal agencies to demonstrate effectiveness of eDNA methodology for biological-monitoring of freshwater systems.
