These data are comprised of measurements of metals (cobalt, nickel, copper, zinc, cadmium, and lead), anions (fluoride, chloride, nitrite, bromide, nitrate, sulfate, and phosphate), dissolved organic carbon, and major cations (sodium, magnesium, potassium, calcium, manganese, iron, and strontium) in surface and pore water samples collected during an assessment of the impacts of mining-related metals on the keystone crayfish species, Orconectes quadruncus, in the St. Francois draininge in Madison County, Missouri USA.