This dataset provides an estimate of aquatic biodiversity sites, based on protected and conservation lands superimposed on the NHD flowlines dataset. Caution is warrented in interpreting this dataset. While measures of aquatic biodiversity exist, they are based on localized samples that cannot be extrapolated beyond the sampling domain. These measures were not included in this dataset. Instead, it was assumed that areas within TNC Portfolio areas were identified for factors that include high aquatic biodiversity. Furthermore, it is assumed that aquatic biodiversity is higher in protected areas due to stricter controls over land and water management. However, this assumption may not be valid for stream reaches that flow through protected [...]