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Conversion and fragmentation of wildlife habitat often leads to smaller and isolated populations and can reduce a species’ ability to disperse across the landscape. As a consequence, genetic drift can quickly lower genetic variation and increase vulnerability to extirpation. For species of conservation concern, quantification of population size and connectivity can clarify the influence of genetic drift in local populations and provides important information for conservation management and recovery strategies. Here, we used genome-wide single nucleotide polymorphism (SNP) data and capture-mark-recapture methods to evaluate the population structure, genetic diversity and abundance of seven focal sites of the endangered...
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Fragmentation and loss of natural habitat have important consequences for wild populations and can negatively affect long-term viability and resilience to environmental change. Salt marsh obligate species, such as those that occupy the San Francisco Bay Estuary in western North America, occupy already impaired habitats as result of human development and modifications and are highly susceptible to increased habitat loss and fragmentation due to global climate change. We examined the genetic variation of the California Ridgway’s rail ( Rallus obsoletus obsoletus), a state and federally endangered species that occurs within the fragmented salt marsh of the San Francisco Bay Estuary. We genotyped 107 rails across 11...
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To understand the genetic structure of six rare plant species (Acanthomintha ilicifolia, Baccharis vanessae, Chloropyron maritimum ssp. maritimus, Deinandra conjugens, Dicranostegia orcuttiana, Monardella viminea), we obtained samples from known occurrences in San Diego County, prepared and sequenced ddRAD genomic libraries, and developed and analyzed a panel of genetic markers to assess population diversity and divergence. We provide this SNP genotype dataset in Structure format, produced in Stacks v2.0b8, for future monitoring efforts and population genetic analysis. These data support the following publication: Milano, E.R., and Vandergast, A.G., 2018, Population genomic surveys for six rare plant species in...
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Capture-mark-recapture data from San Francisco Gartersnakes at five sites in San Mateo County, California in 2018. These data include capture histories, snout-vent lengths, and sex for individual snakes. R files included with the data fit closed capture-mark-recapture models to estimate the abundance of adult snakes at each site in 2018.
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These data represent stem growth from whitebark pine at 27 sites in the Sierra Nevada of California. Values for stem growth were derived from increment cores, processed following standard methods. Samples were also compared against a genomic data collected at the same trees. These data support the following publication: van Mantgem, P.J., Milano, E.R., Dudney, J., Nesmith, J.C.B, Vandergast, A.G., and Zald, H.S.J., 2023. Growth, drought response, and climate-associated genomic structure in whitebark pine in the Sierra Nevada of California. Ecology and Evolution. https://doi.org/10.1002/ece3.10072.
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The Coastal Cactus Wren is a species of high conservation concern in southern California. The goal of this project was to obtain taxonomic resolution of subspecies boundaries between coastal and desert populations of Cactus Wrens. We collected genetic samples from Cactus Wrens at California desert sites and combined this dataset with previously collected samples from coastal populations to resolve the distribution of subspecies and to determine distinct genetic lineages, gene flow, genetic diversity and effective population size of desert populations. Understanding these relationships will assist managers with development and implementation of a conservation and management strategy that could aim to avoid state...
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The data set consists of 12 input data rasters that cover San Diego County, California. These input rasters represent criteria used in a Pareto ranking algorithm in the manuscript. These include three rasters related to fire threats, three rasters related to habitat fragmentation threats, four rasters related to species biodiversity, and two rasters related to genetic biodiversity. (see the PLOS ONE paper for details). These data support the following publication: Tracey JA, Rochester CJ, Hathaway SA, Preston KL, Syphard AD, Vandergast AG, et al. (2018) Prioritizing conserved areas threatened by wildfire and fragmentation for monitoring and management. PLoS ONE 13(9): e0200203. https://doi.org/10.1371/journal.pone.0200203
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Data presented are 1.) the locations where Coastal Cactus Wren (Campylorhynchus brunneicapillus) genetic samples were collected in southern California, in 2011, 2012, and 2013; 2.) 2012 and 2013 survey results; 3.) the territory locations of all Cactus Wrens detected in 2011, 2012, and 2013 in Orange, Riverside, and San Diego counties; and 4.) dispersal results on a subset of Cactus Wrens color banded in 2011. These data support the following: Barr, K.R., Kus, B.E., Preston, K.L., Howell, S., Perkins, E. and Vandergast, A.G., 2015. Habitat fragmentation in coastal southern California disrupts genetic connectivity in the cactus wren (Campylorhynchus brunneicapillus). Molecular Ecology, 24(10), pp.2349-2363.
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These data show the multilocus genotypes, gender, and midline carapace length (MCL) for desert tortoises (Gopherus agassizii) sampled in the central portion of the Mojave desert tortoise range. This data supports the following publication: Dutcher, K.E., Vandergast, A.G., Esque, T.C., Mitelberg, A., Matocq, M.D., Heaton, J.S. and Nussear, K.E., Genes in space: what Mojave Desert tortoise genetics can tell us about landscape connectivity. Conservation Genetics, pp.1-15.
The Mogollon Narrowheaded gartersnake (Thamnophis rufipunctatus) and the Northern Mexican gartersnake (Thamnophis eques megalops) are both listed as Threatened under the Federal Endangered Species Act. Both species have a strong association with aquatic habitats, and these habitats have been highly altered from impoundments, land-use changes, and the introduction and spread of non-native aquatic species, which have contributed to declines in Arizona and New Mexico over the last 30-40 years. We characterized 125 microsatellite loci per species to generate genetic toolsets for use in long term genetic monitoring of populations. We evaluated microsatellite loci for missing data and several variability thresholds, which...
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Genetic diversity within species provides the raw material for adaptation and evolution. Just as regions of high species diversity are conservation targets, identifying regions containing high genetic diversity and divergence within and among populations may be important to protect future evolutionary potential. When multiple co-distributed species show spatial overlap in high genetic diversity and divergence, these regions can be considered evolutionary hotspots. We analyzed genetic data from a variety of vertebrate and invertebrate species distributed across the Mojave Desert to map regions of high genetic diversity and divergence. These genetic landscapes can be analyzed with respect to current and proposed development...
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Microsatellite data formatted for the software CONVERT, which includes dataset numerical IDs, sampling site names, and locus identifiers. Rows 291-294 provides information about the allele size ranges and repeat motif for each locus. Missing data are coded as ?.
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Genetic samples in the form of a small blood sample were collected from 263 Bell’s vireos (Vireo bellii) in drainages throughout southern and central California, Arizona, southern Nevada, and southwestern Utah. We used population genomic techniques to delineate the range limit between the Least Bell’s Vireo (V. b. pusillus) and its conspecific geographical neighbor, the Arizona Bell’s Vireo (V. b. arizonae). This study provides a taxonomic classification of individuals sampled where the two subspecies meet. Moreover, it allows an estimate of genetic diversity and trends in both taxa, estimates sources and levels of dispersal and gene flow among drainages, and allows us to assess progress of recovery and effectiveness...
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Multiple large scale solar, wind, and geothermal energy development projects are currently proposed across the Mojave and Sonoran deserts of the southwestern United States, and these development needs are likely to continue or increase into the future. Agencies tasked with managing biological resources throughout this region must understand the potential impacts of these renewable energy plants and associated infrastructure (e.g., transmission corridors, substations, access roads, etc.) in order to select appropriate development sites and to mitigate for anticipated effects. One of the major concerns of this new green energy initiative is determining how future development will impact wildlife movement, population...
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These data show the multilocus genotypes, as well as extraction and sample genotype quality assessments for desert tortoise (Gopherus agassizii) scat samples. These data support the following publication: Mitelberg, A., Vandergast, A.G., Nussear, K.E., Dutcher, K. and Esque, T.C., 2019. Development of a Genotyping Protocol for Mojave Desert Tortoise Scat. Chelonian Conservation and Biology. https://doi.org/10.2744/CCB-1394.1.
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This dataset contains 212 samples from 8 Oenothera deltoides subsp. howellii occurrences, 3 occurrences of the unknown taxon- clade X, and 3 occurrences of related Oenothera deltoides subsp. cognata. Genomic markers were generated from ddRAD loci (Peterson and others, 2012) and analyzed using the Stacks v2.53 (Catchen and others, 2013) pipeline. The genotypes for all samples are provided in a VCF file with 2,693 independent loci and 15 percent missing data. A companion file is provided with sample names and occurrence designations. These files may be opened and edited in a text editor program, such as Notepad (PC) or BBEdit (Mac). The .vcf file can be loaded into the Stacks population program (Catchen and others,...
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Microsatellite STRUCTURE input file that corresponds with the samples in the ‘refSampleLoc’ worksheet. Missing data are coded as -999.
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A new analysis approach in landscape genetics and phylogeography is the creation of “genetic landscapes” to visualize genetic structure across geographic space. The Genetic Landscapes GIS Toolbox contains four tools to map genetic landscapes and to summarize multiple genetic landscapes as average and variance surfaces in ArcGIS ® (Environmental Science Research Institute, Redlands, CA, USA). Together, these tools automate a series of calculations and data manipulations to create genetic landscape surfaces directly from tables containing genetic distance or diversity data and sample location coordinates. This allows users with little GIS experience to create and analyze these complex raster surfaces with efficiency...
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These data show multilocus genotypes, banding age, and territory for coastal cactus wrens (Campylorhynchus brunneicapillus) sampled in coastal southern California between 2009 and 2019. These data support the following publication: Vandergast, A.G., Kus, B.E., Smith, J.G. and Mitelberg, A., 2022. Recent declines in genetic diversity with limited dispersal among coastal cactus wren populations in San Diego County, California. Conservation Science and Practice, 4(9), p.e12780. https://doi.org/10.1111/csp2.12780.


map background search result map search result map Species On Lands Affected by Renewables (SOLAR) Mojave Landscape Genetics Study A Century of Landscape Disturbance and Urbanization of the San Francisco Bay Region affects the Present-day Genetic Diversity of the California Ridgway’s Rail (Rallus obsoletus obsoletus) Microsatellite CONVERT Input File Microsatellite STRUCTURE Input File Distribution and Population Genetic Structure of Coastal Cactus Wrens in Southern California Genotypes for six rare plant species found in San Diego County in 2016-2017 Raster data files for “Prioritizing conserved areas threatened by wildfire for monitoring and management." Microsatellite genotypes for desert tortoise (Gopherus agassizii) from scat (2016-2018) Microsatellite genotypes for desert tortoise (Gopherus agassizii) in Ivanpah Valley (2015-2017) Oenothera deltoides Genotype Data from Contra Costa County, California in 2019 Growth, Drought Response, and Genomic Structure Data for Whitebark Pine in the Sierra Nevada of California (ver. 2.0, May 2023) San Francisco Gartersnake (Thamnophis sirtalis tetrataenia) Genomic and Demographic Data from San Mateo County and Northeastern Santa Cruz County Collected Between 2016 - 2018 Capture-mark-recapture Data for San Francisco Gartersnakes in San Mateo County, California, 2018 Microsatellite Loci for Mogollon Narrowheaded Gartersnake (Thamnophis rufipunctatus) and the Northern Mexican Gartersnake (Thamnophis eques megalops) in Arizona and New Mexico (2020-2021) Microsatellite Genotypes for Coastal Cactus Wrens (Campylorhynchus brunneicapillus) from Southern California, 2009-2019 Assessing Connectivity among Coastal and Interior Cactus Wrens in California using Population Genomics (2021) Capture Data and SNP Genotypes to Evaluate Subspecies Range Boundaries of the Bell’s vireo (Vireo bellii pusillus and V. b. arizonae) (2010-2022) A Century of Landscape Disturbance and Urbanization of the San Francisco Bay Region affects the Present-day Genetic Diversity of the California Ridgway’s Rail (Rallus obsoletus obsoletus) Microsatellite genotypes for desert tortoise (Gopherus agassizii) in Ivanpah Valley (2015-2017) Oenothera deltoides Genotype Data from Contra Costa County, California in 2019 Capture-mark-recapture Data for San Francisco Gartersnakes in San Mateo County, California, 2018 Genotypes for six rare plant species found in San Diego County in 2016-2017 Raster data files for “Prioritizing conserved areas threatened by wildfire for monitoring and management." Distribution and Population Genetic Structure of Coastal Cactus Wrens in Southern California Microsatellite genotypes for desert tortoise (Gopherus agassizii) from scat (2016-2018) Microsatellite Genotypes for Coastal Cactus Wrens (Campylorhynchus brunneicapillus) from Southern California, 2009-2019 Growth, Drought Response, and Genomic Structure Data for Whitebark Pine in the Sierra Nevada of California (ver. 2.0, May 2023) Assessing Connectivity among Coastal and Interior Cactus Wrens in California using Population Genomics (2021) Species On Lands Affected by Renewables (SOLAR) Microsatellite Loci for Mogollon Narrowheaded Gartersnake (Thamnophis rufipunctatus) and the Northern Mexican Gartersnake (Thamnophis eques megalops) in Arizona and New Mexico (2020-2021) Microsatellite CONVERT Input File Microsatellite STRUCTURE Input File San Francisco Gartersnake (Thamnophis sirtalis tetrataenia) Genomic and Demographic Data from San Mateo County and Northeastern Santa Cruz County Collected Between 2016 - 2018 Capture Data and SNP Genotypes to Evaluate Subspecies Range Boundaries of the Bell’s vireo (Vireo bellii pusillus and V. b. arizonae) (2010-2022) Mojave Landscape Genetics Study