Filters: Contacts: Eric Leis (X)
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The diagnosis of bacterial disease in freshwater unionid mussels has been hindered by a lack of baseline information regarding the microbial communities associated with these animals. In this study, we cultured and identified bacteria from the hemolymph of stable mussel populations from the upper Mississippi River basin and compared results to mussel populations associated with a mortality event in the Clinch River, VA and TN. Several bacterial genera were consistently identified across mussel species and locations, appearing to be part of the natural bacterial flora. One noteworthy isolate was identified from the Clinch River. Yokenella regensbergei was found with relatively high prevalence during the mortality...
Categories: Data;
Tags: Bacteria,
Clinch River, Virginia, USA,
Clinch River,Tennessee, USA,
Hemolymph,
USGS Science Data Catalog (SDC),
This dataset uses the ITIS recommended Ortmanniana pectorosa, Conrad, 1834 in place of Actinonaias pectorosa, which was used in the Richard et al. 2020 manuscript. Biologists monitoring freshwater mussel (Order Unionida) populations rely on behavioral, often subjective, symptoms to identify “sick” or stressed animals, such as gaping valves and slow response to probing and lack clinical indicators to support a diagnosis. As part of a multi-year study to investigate causes of reoccurring mortality of pheasantshell (Ortmanniana pectorosa, Conrad 1834) in the Clinch River, Virginia and Tennessee, USA, we analyzed the hemolymph metabolome of a subset of mussels from the 2018 sampling period. Mussels at the mortality...
Categories: Data;
Tags: Aquatic Biology,
Clinch River, VA, USA,
USGS Science Data Catalog (SDC),
Wildlife Biology,
biota
This study evaluated the use of medicated feeds to treat furunculosis infections caused by the bacterium Aeromonas salmonicida in lake herring (Coregonus artedi, Lesueur, 1818). The objectives of this two-phase study were to (1) determine if Coregonus artedi could be adequately and consistently infected with A. salmonicida by the methods of bath immersion or intraperitoneal (IP) injection, and (2) determine if the medicated feeds Oxytetracycline Dihydrate (Terramycin® 200 For Fish) and Florfenicol (Aquaflor®) could be used for the control of furunculosis caused by A. salmonicida. These datasets include water quality, mortality, feed weights and behavior, and qPCR data collected during both phases of the study.
This was a joint project between: Upper Midwest Environmental Sciences Center, U.S. Geological Survey, La Crosse, WI 54603 La Crosse Fish Health Center, U.S. Fish and Wildlife Service, Onalaska, WI 54650 This repository contains the raw data and R codes used in the metabolomics analysis for the manuscript which used silver and bighead carp fry cells exposed to thiram. Species indclude in this manuscript Silver Carp (Hypophthalmichthys molitrix) Bighead Carp (Hypophthalmichthys nobilis) The raw data is in: BHC thiram data.csv SVC thiram data labels.csv The code used in the ordination analysis is in: figure_1_knit_code.Rmd The code used in the principle component analysis is in: figure_2_knit_code.Rmd The...
Categories: Data
We investigated the cultureable bacterial communities in the native freshwater mussel faunal in the Clinch River, VA/TN during mussel mortality events in 2018, 2019 and 2020 and examine the spatial and temporal distribution of bacterial genera among Pheasantshells and six other unionid species.
Categories: Data;
Tags: Aquatic Biology,
Clinch River,
Tennessee,
USGS Science Data Catalog (SDC),
Virginia,
During an epidemiologic survey following a mortality event of freshwater mussels in 2018 in the Embarrass River, Wisconsin, USA, we identified a novel microsporidian parasite in the ovary of mucket (Actinonaias ligamentina), plain pocketbook (Lampsilis cardium), and fatmucket (Lampsilis siliquoidea) (Unioinidae). Histopathology showed round-to-oval microsporidial spores in the cytoplasm of oocytes in 60% (3/5) of mucket, 100% (4/4) of plain pocketbook and 50% (1/2) of fatmucket. On transmission electron microscopy, mature spores were round to oval, measured 4.13 +/- 0.64 µm (3.14–5.31) long by 2.88 +/-0.37 µm (2.36–3.68) wide. Spores had a thin electron dense exospore with a spiky coat, a thick electron lucent endospore,...
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