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Folders: ROOT > ScienceBase Catalog > Chesapeake Bay Endocrine Disrupting Chemicals ( Show all descendants )
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We have adapted two commonly used human breast luciferase transactivation cell bioassays, the recently re-named estrogen agonist/antagonist screening VM7Luc4E2 cell bioassay (previously designated BG1Luc4E2) and the androgen/glucocorticoid screening MDA-kb2 cell bioassay, to 384-well formats for HTS of endocrine-active substances. This cost-saving adaptation includes a fast, accurate, and easy measurement of protein amount in each well via the fluorescamine assay (in the form of relative fluorescence units, RFU which are then converted to microgram protein using bovine serum albumin standards) with which to normalize luciferase activity (in the form of relative light units, RLU) of cell lysates without requiring...
Categories: Data;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Columbia Environmental Research Center,
Missouri,
USGS Science Data Catalog (SDC),
bioassay,
cells,
Incedental wastewater reuse from streams that receive discharges from Wastewater Treatment Facilities (WWTF) has the potential to be a significant contributor of Endocrine Disrupting Chemicals. An ArcGIS model of WWTFs, NHDPlus Version 2 stream networks (USGS and EPA 2012), and gage stations across the Shenandoah River watershed was created to calculate accumulated wastewater in percent of streamflow (ACCWW%) and Predicted Environmental Concentrations (PECs) of select constituents. Virginia and West Virginia Pollutant Discharge Elimination System (VPDES, WVPDES) discharge facilities, outfall locations, and stream gages were spatially joined to the nearest river segment. Wastewater inputs from outfall locations were...
Categories: Data Release - Revised;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Aquatic Biology,
Ecology,
Environmental Health,
Shenandoah River,
USGS Science Data Catalog (SDC),
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