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Location Folder Show Tree ROOT _ScienceBase Catalog __Chesapeake Bay Endocrine Disrupting Chemicals Filters Date Range Past year (0) Past month (0) Past week (0) Past day (0) Custom range... To   Search Types Map Service (2) OGC WFS Layer (2) OGC WMS Service (2) Contacts Jennifer C Brennan Chintamani Kandel Virginia and West Virginia Water Science Center Columbia Environmental Research Center U.S. Geological Survey More... Categories Data (1) Data Release - Revised (1) Tag Types Theme (2) USGS Scientific Topic Keyword (1) Place (2) Harvest Set (2) Tag Schemes None (2) Common geographic areas (2) Marine Realms Information Bank (MRIB) keywords (1) USGS Thesaurus (1) View Results as: JSON ATOM CSV Browse Folder

Development of a dual luciferase-fluorescamine assay adapted to a 384 micro-well plate format-Data We have adapted two commonly used human breast luciferase transactivation cell bioassays, the recently re-named estrogen agonist/antagonist screening VM7Luc4E2 cell bioassay (previously designated BG1Luc4E2) and the androgen/glucocorticoid screening MDA-kb2 cell bioassay, to 384-well formats for HTS of endocrine-active substances. This cost-saving adaptation includes a fast, accurate, and easy measurement of protein amount in each well via the fluorescamine assay (in the form of relative fluorescence units, RFU which are then converted to microgram protein using bovine serum albumin standards) with which to normalize luciferase activity (in the form of relative light units, RLU) of cell lysates without requiring... Categories: Data; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Columbia Environmental Research Center, Missouri, USGS Science Data Catalog (SDC), bioassay, cells, All tags... fluorescence, luciferase, Fewer tags Shenandoah River Accumulated Wastewater Ratio Incedental wastewater reuse from streams that receive discharges from Wastewater Treatment Facilities (WWTF) has the potential to be a significant contributor of Endocrine Disrupting Chemicals. An ArcGIS model of WWTFs, NHDPlus Version 2 stream networks (USGS and EPA 2012), and gage stations across the Shenandoah River watershed was created to calculate accumulated wastewater in percent of streamflow (ACCWW%) and Predicted Environmental Concentrations (PECs) of select constituents. Virginia and West Virginia Pollutant Discharge Elimination System (VPDES, WVPDES) discharge facilities, outfall locations, and stream gages were spatially joined to the nearest river segment. Wastewater inputs from outfall locations were... Categories: Data Release - Revised; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Aquatic Biology, Ecology, Environmental Health, Shenandoah River, USGS Science Data Catalog (SDC), All tags... Virginia, Water Quality, Water Resources, inland waterways, wastewater discharge, water, wastewater, hydrology, streamflow, Fewer tags