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The data include concentrations of current use pesticides in tissues of larval wood frog (Lithobates sylvaticus) and spotted salamander (Ambystoma maculatum) and the presence of ranavirus in wood frogs and spotted salamanders from three northeastern National Wildlife Refuges sampled in 2013 and 2014. The data also include estrogenicity, protein phosphatase 2A inhibition and a suite of 15 major and minor elements in sediment screened using portable X-Ray Fluorescence. The data include sediment and tissue samples collected from 16 wetlands at the Patuxent Research Refuge (PRR) in central Maryland, USA, 15 wetlands at the Assabet River and Oxbow National Wildlife Refuges (EMASS) in eastern Massachusetts, USA, and nine...
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The data included here were gathered to determine the effects of cattle grazing on wetland water quality in the Klamath National Wildlife Refuge in Oregon. Surface water samples from sites representing a range of grazing histories were collected between June and October in 2017 and 2018. Samples were analyzed for nutrients (ammonia, nitrate plus nitrite, nitrite, and orthophosphate), total coliforms, E. coli, enterococci, and estrogenicity by U.S. Geological Survey laboratories. Basic water quality parameters such as temperature, pH, turbidity and specific conductance were also collected in the field during each site visit. Quality assurance samples (blanks and replicates) as well as method information is also included...
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The data were gathered as part of a long-term, seasonal assessment of estrogenicity in selected locations within Pennsylvania from 2012-2017. Data were collected over this time frame to determine seasonal and annual changes in surface water estrogenicity.Estrogen is reported as estrogen equivalents (EEQ) as determined by a bioluminescent yeast estrogen screen. A total of 193 grab water samples were collected during this time of which 41% had EEQ above the limit of detection. The maximum estrogen equivalents (EEQ) measured was 5.44 ng EEQ/ L, while the median concentration was 0.44 ng/L. POCIS samplers were deployed at a number of sites to determine time-weighted estrogenicity between 2014 and 2017. A total of 150...
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The data presented in this data release includes 11 field/water quality parameters, concentrations of 16 nutrients/anions, dissolved organic carbon, 14 organic contaminants, net estrogenicity concentrations, and 51 inorganic constituents in surface water collected twice from 28 stream sites and collected once from 2 stream sites in the Shenandoah Valley (Virginia and West Virginia, USA), in the summer of 2021 (Table_1_Sites and Table_2_Methods). The estrogenicity, inorganic, and organic chemical characteristics of river waters were analyzed using 19 separate analytical methods at 5 laboratories (Table_2_Methods). Surface water was analyzed for water quality and nutrients (Table_3_WQ_Nutrients_Anions_DOC), toxic...
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In the spring of 2021, six households with private wells were selected throughout Graton, California, based on proximity to agriculture (within one mile of agriculture), well depth (i.e., less than 150 feet in depth), and well type (i.e., dug versus drilled). Silicone bands were deployed for 30 days at outdoor locations at each household to passively sample pesticides in ambient air. On May 3, 2021, the silicone bands were retrieved and outdoor water samples (i.e., spigot on the side of the house) and indoor tap water samples (i.e., kitchen faucet used for drinking water) were collected and analyzed for 187 pesticides, 57 potential hormonally active chemicals, and estrogenicity. This study was a citizen science...
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Data were collected as part of an investigation developed by Leetown Science Center to investigate the comparative detection of avian influenza viruses from waterfowl and potential environmental reservoirs such as aquatic sediment from waterfowl habitat. This dataset identifies positive or negative test results for qRT-PCR (quantitative reverse transcriptase polymerase chain reaction) for avian influenza virus through identification of the Type A influenza virus matrix gene from aquatic sediment samples. Sediment samples were collected from waterfowl habitat so as to determine if temporal and spatial differences in virus detection by qRT-PCR were evident among test sites.
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This data set includes information pertaining to 300 bivalve mollusk tissue samples collected from mollusk specimens from waters in eastern Maryland on the Delmarva Peninsula. Data includes source location, species, morphometric data, and IAV matrix gene detection status for the bivalve samples collected and analyzed in the associated study.
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The files in this data release are the processed DNA consensus sequence alignment files for the chelonid herpesvirus 5 (ChHV-5) genome plus 66 open reading frames representing protien-coding genes. DNA was extracted from tumor biopsies from green sea turtles (Chelonias mydas) with fibropapillomatosis sampled from Florida and Hawaii coastal waters. We used high-throughput short-read sequencing (Illumina MiSeq and Ion Torrent Personal Genome Machine) of long-range PCR products amplified from tumor tissue using primers designed from the single available ChHV5 reference genome from a Hawaii green sea turtle. The raw sequence data is available at the NCBI Sequence Read Archive under bioproject number PRJNA360405, including...
Tags: Class Reptilia, DNA sequencing, Earth, Oceans, Atlantic, North Atlantic, Earth, Oceans, Pacific, Family Cheloniidae, All tags...
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Disease outbreaks, skin lesions, fish kill events, and reproductive abnormalities have been observed in wild populations of bass in the Chesapeake Bay watershed. Occurrence of synthetic and natural hormones from wastewater treatment plants and livestock operations, pesticides from agricultural lands, and phytoestrogens from cover crops have been implicated as potential causes of these adverse effects. Late summer to fall is the period of early gonad recrudescence in adult bass when spermatogenesis and oogenesis begin for the upcoming spawning event in spring. Our objective was to assess whether early gonad recrudescence was a period of sensitivity for inducing immunomodulation. Adult largemouth bass (LMB; Micropterus...
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Oklahoma State University South-Central Research Station (SCRS) was used to conduct research to understand the chemical composition of various water types and their potential environmental and human health effects. The study area provided the opportunity to study five water types: (1) receiving surface water (Washita River), (2) urban stormwater, (3) wastewater treatment plant effluent used for irrigation, (4) rain-induced runoff from an effluent-irrigated cornfield, and (5) rain-induced runoff from a dryland cornfield. Fifteen water samples were collected from August 2018 to October 2019. In addition, two composite above ground whole-corn plant samples (i.e., stem, leaves, grain) were collected from the effluent...
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This data release contains coastal wetland synthesis products for the geographic region of north shore Long Island, New York. Metrics for resiliency, including unvegetated to vegetated ratio (UVVR), marsh elevation, and mean tidal range, are calculated for smaller units delineated from a Digital Elevation Model, providing the spatial variability of physical factors that influence wetland health. Through scientific efforts initiated with the Hurricane Sandy Science Plan, the U.S. Geological Survey has been expanding national assessment of coastal change hazards and forecast products to coastal wetlands with the intent of providing Federal, State, and local managers with tools to estimate the vulnerability and ecosystem...
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Samples were collected, processed, and analyzed for organics, estrogen equivalents, and fecal indicator bacteria. Filtered organic samples were sent to the National Water Quality Laboratory in Denver, Colorado. Unfiltered estrogen equivalent samples were sent to the Organic Geochemistry Research Lab in Lawrence, Kansas, for extraction, after which they were sent to the National Fish Health Research Laboratory in Leetown, West Virginia. Bacteria samples were processed at the Central-Midwest Water Science Center Iowa City, Iowa, office. Staff collected field parameters in-situ.
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A large spill of wastewater from oil and gas operations was discovered adjacent to Blacktail Creek near Williston, North Dakota in January 2015. To determine the effects of this spill on streambed microbial communities over time, bed sediment samples were taken from Blacktail Creek upstream, adjacent to, and at several locations downstream from the spill site. Blacktail Creek is a tributary of the Little Muddy River, and additional samples were taken upstream and downstream from the confluence of Blacktail Creek and the Little Muddy River. Samples were collected in February 2015, June 2015, June 2016, and June 2017. DNA was extracted from these sediments, and sequencing of the 16S ribosomal RNA gene was performed...
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Intersex in small- and largemouth bass has been observed in the upper Chesapeake Bay Watershed since 2003. This observation is indicative of exposure to estrogenic endocrine disrupting chemicals. In order to complement biological data sets and better identify the spatial and temporal nature of this end-point, discrete grab water samples were collected throughout the upper Chesapeake Bay Watershed for the evaluation of estrogenic activity. Data reported here are estrogenicity values derived from bioassay screening of OASIS-HLB extracts discrete grab water samples. Estrogenicity was determined using a bioluminescent yeast estrogen screen. Here we report estrogenicity data for selected sites in the Chesapeake Bay water...
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The data were gathered as a preliminary assessment of estrogenicity under base-flow conditions at over 100 sites (lakes and streams) throughout New Jersey followed by more targeted sampling of smallmouth bass at nine sites with varying levels of estrogenicity. In 2016, 102 sites (lakes and streams) for the preliminary estrogenicity assessment were selected utilizing compiled results of previous monitoring studies (chemical and biological), current sampling networks, and other areas of concern based on input from stakeholders. Discrete grab surface water samples were collected under base-flow conditions in the fall of 2016 and analyzed for levels of estrogenicity using a bioluminescent yeast screen. Water samples...


    map background search result map search result map Estrogen Equivalents of Surface Water in the Upper Chesapeake Bay Watershed (2008 - 2011) Targeted amplicon resequencing of chelonid alphaherpesvirus 5 from tumor samples collected from Chelonia mydas (green turtle) inhabiting Hawaiian and Florida coastal waters Molecular detection of avian influenza virus from sediment samples in waterfowl habitats on the Delmarva Peninsula, USA Estrogen equivalents of surface water and smallmouth bass estrogenic biomarker data in New Jersey, 2016-2017 Influenza A virus detected from bivalves in waterfowl habitat of the Delmarva Peninsula Nutrients, estrogenicity, and fecal indicators in surface water collected from wetlands in the Klamath Marsh National Wildlife Refuge, Oregon, 2017-2018 Exposure potential of marsh units to environmental health stressors in north shore Long Island salt marsh complex, New York Water-quality data for a pharmaceutical study at Muddy Creek in North Liberty and Coralville, Iowa, 2017-2018 Current use pesticides in larval amphibian tissues, amphibian pathogen and wetland sediment screening data from three northeastern National Wildlife Refuges, 2013-14 Microbial Community Composition Data from Blacktail Creek near Williston, North Dakota Water-quality results from a wastewater reuse study: Inorganic and organic compositions of wastewater effluent and select urban and agricultural water types during rain-induced runoff, Chickasha, Oklahoma, 2018-2019 Estrogen equivalents of surface water in Pennsylvania, 2012-2017 Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Water quality and contaminants in stream surface waters collected in the Shenandoah Valley, 2021 Results from U.S. Geological Survey Environmental Health Food Resources Lifecycle Integrated Science Team, Graton Pesticides (GRAPE) Study, in Graton, California, April–May 2021 Effects of estrogens and atrazine on functional immune responses of largemouth bass (Micropterus salmoides) Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Effects of estrogens and atrazine on functional immune responses of largemouth bass (Micropterus salmoides) Water-quality data for a pharmaceutical study at Muddy Creek in North Liberty and Coralville, Iowa, 2017-2018 Nutrients, estrogenicity, and fecal indicators in surface water collected from wetlands in the Klamath Marsh National Wildlife Refuge, Oregon, 2017-2018 Molecular detection of avian influenza virus from sediment samples in waterfowl habitats on the Delmarva Peninsula, USA Exposure potential of marsh units to environmental health stressors in north shore Long Island salt marsh complex, New York Influenza A virus detected from bivalves in waterfowl habitat of the Delmarva Peninsula Results from U.S. Geological Survey Environmental Health Food Resources Lifecycle Integrated Science Team, Graton Pesticides (GRAPE) Study, in Graton, California, April–May 2021 Estrogen equivalents of surface water and smallmouth bass estrogenic biomarker data in New Jersey, 2016-2017 Water quality and contaminants in stream surface waters collected in the Shenandoah Valley, 2021 Estrogen equivalents of surface water in Pennsylvania, 2012-2017 Water-quality results from a wastewater reuse study: Inorganic and organic compositions of wastewater effluent and select urban and agricultural water types during rain-induced runoff, Chickasha, Oklahoma, 2018-2019 Current use pesticides in larval amphibian tissues, amphibian pathogen and wetland sediment screening data from three northeastern National Wildlife Refuges, 2013-14 Estrogen Equivalents of Surface Water in the Upper Chesapeake Bay Watershed (2008 - 2011) Targeted amplicon resequencing of chelonid alphaherpesvirus 5 from tumor samples collected from Chelonia mydas (green turtle) inhabiting Hawaiian and Florida coastal waters