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This data release provides the georeferenced boundaries that delimit each spatial unit of the Great Lakes Regional Aquatic Gap Analysis Project (GLGap) Coastal Hydrospatial Framework at each spatial scale from the local 90m cell to the entire Laurentian Great Lakes system and from the shoreline to the deepest offshore waters. The U.S. Geological Survey and the U.S. Fish and Wildlife Service collaborated to design a universal framework of spatial areas that encompass all space of the Laurentian Great Lakes proper. Agglomeration of the finest units (90m cells) form coarser, broader scale units. Finer units are nested within the coarser units at six spatial scales, labeled as Local Cells, Aquatic Habitat Areas, Coastal...
Field observations in a Lake Ontario embayment suggested that there is a phototaxic response of larval cisco that changes with development. This switch was verified using cultured cisco in a laboratory environment. These data include counts of cisco larvae at three depth ranges within multiple 95 liter tanks. Each tank of larvae were exposed to one of three different light treatments with three replicate tanks per treatment. Light treatments were 24 hours per day of light exposure, 24 hours per day in darkness, and a 12-hour light then 12-hour dark exposure cycle. Observations of cisco positioning within the water column were recorded periodically. The information collected included the date and time of the observation,...
This data set includes the sample site locations, site descriptions, and abiotic data used in the analyses reported in McKenna et al. 2008. The data represent selected abiotic conditions within the St. Lawrence and lower reaches of four US tributaries during 2004. Samples were collected from shallow, seinable areas (< 1.5 m) of the St. Lawrence River, adjacent wetland channels, and the lower reaches of major tributaries (Grass River, Raquette River, St. Regis River, and Salmon River). This part of the St. Lawrence River is non-tidal fresh water. Sample locations were randomly selected from a grid of spatial cells (1 km x 1 km) encompassing the area within the 5,700 ha Akwesasne Wetland Complex (at the intersection...
Taxonomic composition of environmental DNA acquired by filtration from the St. Regis River, New York
Environmental DNA (eDNA) surveys have become important tools for monitoring aquatic biodiversity. Barcode sequencing of eDNA generates community profiles that, while potentially biased in both capture and amplification, can nonetheless yield high information content per unit cost. While factors affecting eDNA capture and amplification have been heavily studied, watershed-scale assessments of fish communities and our confidence in such have been less frequent. We performed an initial watershed-scale characterization of fish eDNA using rapid, low-volume filtering with replicate and control samples scaled for a single Illumina MiSeq flow cell, using the mitochondrial 12S ribosomal RNA locus for taxonomic profiling....
Location, occurrence, collection information, and genetic species identification data in support of an analysis of the spatial separation of Lake Whitefish and Cisco larvae in Chaumont Bay, Lake Ontario, the last known spawning area of both species in American waters of Lake Ontario. Genetic species identification of larval coregonines collected between April 2004 and 2015 is provided for larval samples collected from nearshore and offshore areas.
These data include survival information for Cisco in the laboratory for up to 30 days after surgical implantation of two different sizes of Lotek acoustic tags. Three-hundred fish of a range of sizes were used for the implantation and controls of this experiment, which was repeated three time. Ancillary data of surgical condition, recovery, and time until death or tag expulsion were also recorded, as well as sizes at beginning and end of observation period and estimated growth.
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