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Macrhybopsis reproduction and propagule traits were studied in the laboratory using two temperature regimes and three hormone treatments and which methods produced the most spawns. Only sicklefin chub (M. meeki) spawned successfully although sturgeon chub (M. gelida) released unfertilized eggs. All temperature and hormone treatments produced M. meeki spawns, but two treatments had similar success rates at 44 and 43%, consisting of a constant daily temperature with no hormone added, or daily temperature fluctuations with hormone added to the water. Spawns consisted of multiple successful demersal circular swimming spawning embraces interspersed with circular swims without embraces. The most spawns observed for one...
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The Columbia Environmental Research Center (CERC) is a U.S. Geological Survey (USGS) environmental science research facility located in Columbia, Missouri, USA. CERC conducts national and international environmental contaminant research and investigates effects of habitat alterations on aquatic and terrestrial ecosystems.
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This data was generated from a study in which five experiments were conducted that tested whether and how dissolved chemicals might assist food recognition in two filter-feeding fishes, the silver (Hypophthalmichthys molitrix) and the bighead carp (H. nobilis). The buccal-pharngeal pumping (BPP), a behavior in which fish pump water into their buccal cavities, was observed in both silver and bighead carps after exposure to a a variety of food filtrates and mixtures. In addition, occlusion experiments to determine if the olfactory sense has a very important, but not exclusive, role in bigheaded carp feeding behaviors were conducted.
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This child data release includes hyperspectral and RGB images acquired from an Unmanned Aircraft System (UAS) during an experiment performed at the USGS Columbia Environmental Research Center, near Columbia, Missouri, on April 2, 2019. The purpose of the experiment was to assess the feasibility of inferring concentrations of a visible dye (Rhodamine WT) tracer from various types of remotely sensed data in water with varying levels of turbidity. Whereas previous research on remote sensing of tracer dye concentrations has focused on clear-flowing streams, the Missouri River is much more turbid and the reflectance signal associated with the sediment-laden water could obscure that related to the presence and amount...
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Point clouds, high resolution digital surface models (DSM), and high resolution orthoimagery of Palmyra Atoll, USA, were generated from aerial 3-color and 5-band imagery collected October 2016 using Unoccupied Aerial Systems (UAS, or "drones"). Data were processed in sections corresponsing to flight areas using photogrammetry to generate three-dimensional point clouds (.las) by identifying pixels from multiple images representing the same object and calculating the x, y, and z coordinates of that object/pixel. Point clouds for each section were processed to create DSMs (.tif) representing the continuous surface of the uppermost reflective surface (i.e., data have not been filtered for vegetation classes or height)....
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We measured ontogenetic changes in routine and maximum swimming speeds of bighead, grass, and silver carp larvae. Daily measurements of routine swimming speed were taken for two weeks post-hatch using a still camera and the LARVEL program, a custom image-analysis software. Larval swimming speed was calculated using larval locations in subsequent image frames and time between images. Using an endurance chamber, we determined the maximum swimming speed of larvae (post gas bladder inflation) for four to eight weeks post-hatch.
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This dataset includes microsomal EROD data from an assay done with liver samples from several fish species that are found in Arizona at sites that are being assessed for PBDE contamination. The data was created in September and October 2016.
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This child data release includes in situ measurements of turbidity and Rhodamine WT dye concentration acquired during an experiment performed at the USGS Columbia Environmental Research Center, near Columbia, Missouri, on April 2, 2019. The purpose of the experiment was to assess the feasibility of inferring concentrations of a visible dye (Rhodamine WT) tracer from various types of remotely sensed data in water with varying levels of turbidity. Whereas previous research on remote sensing of tracer dye concentrations has focused on clear-flowing streams, the Missouri River is much more turbid and the reflectance signal associated with the sediment-laden water could obscure that related to the presence and amount...
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Grass carp, bighead carp, and silver carp spawn in flowing water. Their eggs,and then larvae, develop while drifting. Hydraulic conditions control spawning locations, egg survival, and the downstream distance traveled before the hatched larvae can swim for low velocity nursery habitats. Existing egg drift models simulate the fluvial transport of carp eggs but have limitations in capturing the effect of localized turbulence on egg transport due to inadequate dimensions of hydrodynamics and/or empirical parameterization of river dispersion. We present a three-dimensional Lagrangian particle tracking model that uses fully resolved river hydrodynamics and a continuous random walk algorithm driven by local turbulent...
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Description of Work Determine the relative sensitivity of two species of amphibians (i.e. Wood Frogs Rana sylvatica, Cricket Frogs Acris crepitans blanchardi, American Toad Bufo americana) to exposure to acute and chronic levels of nitrate, nitrite and ammonia. This data will be used to evaluate the current ammonia criteria and for possible inclusion in the development of criteria for nitrite and nitrate.
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We have adapted two commonly used human breast luciferase transactivation cell bioassays, the recently re-named estrogen agonist/antagonist screening VM7Luc4E2 cell bioassay (previously designated BG1Luc4E2) and the androgen/glucocorticoid screening MDA-kb2 cell bioassay, to 384-well formats for HTS of endocrine-active substances. This cost-saving adaptation includes a fast, accurate, and easy measurement of protein amount in each well via the fluorescamine assay (in the form of relative fluorescence units, RFU which are then converted to microgram protein using bovine serum albumin standards) with which to normalize luciferase activity (in the form of relative light units, RLU) of cell lysates without requiring...
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Description of Work The work completed for this template will provide the following information: 1) Generate acute and chronic toxicity data for freshwater mussels and snails to enhance the protection of listed/endangered species in the GL basin; 2) Refine methods and conduct acute and chronic toxicity tests on amphibians; 3) Develop and test methods for conducting chronic toxicity tests for mayflies.
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Advances in drilling technique have facilitated a rapid increase in hydrocarbon extraction from energy shales, including the Williston Basin in central North America. This area overlaps with the Prairie Pothole Region and greater than 35% of wetlands are less than or equal to 1 km from a petroleum-related well. Legacy practices often released saline co-produced waters (brines) that were chloride rich wastes, affecting wetland water quality directly or persisting in sediments. Despite the potential threat of brine contamination to aquatic habitats, there has been little research into their ecological effects. We capitalized on a gradient of legacy brine-contaminated wetlands in northeast Montana to conduct laboratory...
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Invasive crayfish are known to displace native crayfish species, alter aquatic habitat and community structure and function, and are serious pests for fish hatcheries. White River Crawfish (WRC; Procambarus acutus) were inadvertently introduced to a warm-water fish hatchery in Missouri, USA, possibly in an incoming fish shipment. We evaluated the use of chemical control for crayfish to ensure incoming and outgoing fish shipments from hatcheries do not contain live crayfish. We conducted acute (less than or equal to 24 hr) static toxicity tests to determine potency, dose-response, and selectivity of pesticides to WRC, Virile Crayfish (VC; Orconectes virilis), and Fathead Minnow (FHM; Pimephales promelas). Data included...
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Invasive Asian carps established in the United States spawn in turbulent water of rivers and their eggs and early larvae develop while drifting in the current. The eggs are slightly denser than water and are held in suspension by water turbulence. The eggs are believed to perish if they settle before hatching. It is thus possible to use egg drift modeling to assess the capability of a river to support survival of Asian carp eggs. Data to populate such models include the physical properties of the assessed rivers, and information on egg size, density, and terminal fall velocity (sinking rates). Herein, we present the physical characteristics of the eggs as a function of post fertilization time. We recorded mean egg...
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Invasive silver carp (Hypophthalmichthys molitrix) occur in large regions of the Mississippi River drainage, outcompete native species, and are notorious for their prolific jumping behavior. Juvenile and adult (up to 25 kg) carp are known to jump up to 3 m above the water surface in response to moving watercraft. Broadband sound recorded from an outboard motor (100 hp at 32 km/hr) can modulate their behavior in captivity; however, the response of wild silver carp to broadband sound has yet to be determined. This data set includes information regarding the sound produced by outboard motor and speakers in the field and will help in determining effectiveness of the usage of control techniques in the management of carps.
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Management actions intended to increase growth and survival of pallid sturgeon (Scaphirhynchus albus) age-0 larvae on the Lower Missouri River require a comprehensive understanding of the geomorphic habitat template of the river. The datasets described here are intended to support decisions relating to where channel-reconfiguration projects should be located to optimize effectiveness of interception rearing complexes (IRCs). The bend-scale geomorphic classification of the Lower Missouri River is intended to help in the design of monitoring and evaluation of IRC projects. The bend-scale classification was based on geomorphic and engineering variables for 257 bends from Sioux City, Iowa to the confluence with the...
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This child data release includes field spectra and in situ measurements of turbidity and Rhodamine WT dye concentration acquired during an experiment performed at the USGS Columbia Environmental Research Center, near Columbia, Missouri, on April 2, 2019. The purpose of the experiment was to assess the feasibility of inferring concentrations of a visible dye (Rhodamine WT) tracer from various types of remotely sensed data in water with varying levels of turbidity. Whereas previous research on remote sensing of tracer dye concentrations has focused on clear-flowing streams, the Missouri River is much more turbid and the reflectance signal associated with the sediment-laden water could obscure that related to the...
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This data release includes field spectra, UAS-based hyperspectral and RGB images, and in situ measurements of turbidity and Rhodamine WT dye concentration acquired during an experiment performed at the USGS Columbia Environmental Research Center, near Columbia, Missouri, on April 2, 2019. The purpose of the experiment was to assess the feasibility of inferring concentrations of a visible dye (Rhodamine WT) tracer from various types of remotely sensed data in water with varying levels of turbidity. Whereas previous research on remote sensing of tracer dye concentrations has focused on clear-flowing streams, the Missouri River is much more turbid and the reflectance signal associated with the sediment-laden water...


map background search result map search result map Columbia Environmental Research Center (CERC) Determination of the Sensitivity of Two Species of Amphibians to Toxicity From Nitrate, Nitrite and Ammonia Toxicity Testing for Stream Dwelling Amphibians, Mussels, and Mayflies Data in support of manuscript "Evaluation of chemical control for invasive crayfish at a warmwater fish production hatchery" Adaptation and establishment of microchemical methods to analyze elemental patterns in calcified structures of sturgeon for determining origins and movements of freshwater sturgeon in the Mississippi and Missouri river basins Development of a dual luciferase-fluorescamine assay adapted to a 384 micro-well plate format-Data Data for "Comparison of size, terminal fall velocity and density of bighead, silver, and grass carp eggs for use in drift modeling" Microsomal EROD data of fish liver sample assay from species collected in the Salt and Gila Rivers, Arizona Reproductive strategy, spawning induction, spawning temperatures and early life history of captive sicklefin chub Macrhybopsis meeki-Data Widespread legacy brine contamination from oil shales reduces survival of chorus frog larvae-Data Ontogenetic changes in swimming speed of silver carp, bighead carp, and grass carp larvae-Data Chemical cues which include amino acids mediate species-specific feeding behavior in invasive filter-feeding bigheaded carps-Data Asian carp acoustic stimuli data Missouri River bend classification data sets Orthoimagery and elevation data derived from UAS imagery for Palmyra Atoll, USA 2016 (ver. 1.1, November 2020) Field spectra, UAS-based hyperspectral and RGB images, and in situ measurements of turbidity and Rhodamine WT dye concentration from an experiment conducted at the USGS Columbia Environmental Research Center, Columbia, MO, on April 2, 2019 Hyperspectral and RGB images acquired during an experiment conducted at the USGS Columbia Environmental Research Center, Columbia, MO, on April 2, 2019 Field spectra from an experiment conducted at the USGS Columbia Environmental Research Center, Columbia, MO, on April 2, 2019 In situ measurements of turbidity and Rhodamine WT dye concentration from an experiment conducted at the USGS Columbia Environmental Research Center, Columbia, MO, on April 2, 2019 Data supporting a new three-dimensional Lagrangian particle tracking model for predicting transport of carp eggs in turbulent rivers Field spectra from an experiment conducted at the USGS Columbia Environmental Research Center, Columbia, MO, on April 2, 2019 Field spectra, UAS-based hyperspectral and RGB images, and in situ measurements of turbidity and Rhodamine WT dye concentration from an experiment conducted at the USGS Columbia Environmental Research Center, Columbia, MO, on April 2, 2019 Hyperspectral and RGB images acquired during an experiment conducted at the USGS Columbia Environmental Research Center, Columbia, MO, on April 2, 2019 In situ measurements of turbidity and Rhodamine WT dye concentration from an experiment conducted at the USGS Columbia Environmental Research Center, Columbia, MO, on April 2, 2019 Widespread legacy brine contamination from oil shales reduces survival of chorus frog larvae-Data Data supporting a new three-dimensional Lagrangian particle tracking model for predicting transport of carp eggs in turbulent rivers Orthoimagery and elevation data derived from UAS imagery for Palmyra Atoll, USA 2016 (ver. 1.1, November 2020) Missouri River bend classification data sets Adaptation and establishment of microchemical methods to analyze elemental patterns in calcified structures of sturgeon for determining origins and movements of freshwater sturgeon in the Mississippi and Missouri river basins Toxicity Testing for Stream Dwelling Amphibians, Mussels, and Mayflies Determination of the Sensitivity of Two Species of Amphibians to Toxicity From Nitrate, Nitrite and Ammonia