Filters: partyWithName: Wesley A Bickford (X)
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The data document the results of several microbe bioassays performed by the USGS on Phragmites australis plants, including those performed on mature leaves, seedlings, and dead leaf tissues exploration of the literature to find accounts of microbes associated with Phragmites worldwide. For the bioassays, we prepared 162 pure cultures isolated from Phragmites plants in North America along the east coast, Florida, the Gulf of Mexico, and the Great Lakes area, 125 of which were from a previous study, and 38 represent new collections. The DNA sequences used to identify the 37 new collections are included. Microbes were isolated from plants collected from 2015-2018. We performed assays using both North American plant...
To determine how native and non-native lineages of Phragmites australis affect and respond to soil bacteria, fungi and oomycetes, we collected live rhizomes, seeds and soil from native and non-native lineages of Phragmites from 10 sites within Michigan and Ohio, USA. We propagated these field-collected samples to carry out a reciprocal-transplant plant-soil feedback experiment with multiple microbial inhibition treatments. Specifically, we investigated how each Phragmites lineage grew in soils pre-conditioned by each lineage and soils that had been pre-sterilized. Plant biomass was the main response variable collected to determine responses to microbial soil conditioning. We also used DNA meta-barcoding to identify...
To determine the potential for controlling non-native Phragmites australis through use of a cut-to-drown management strategy we performed a controlled greenhouse mesocosm experiment in Ann Arbor, MI during summer 2021. Field collected Phragmites rhizomes from one site within Michigan were propagated, cuttings taken of individual stems and potted in nursery pots. Established plants were then subjected to a complete factorial combination of three submergence treatments (no submergence, partial submergence of aboveground tissues, and compete submergence of aboveground tissues) and 3 cutting treatments (no cutting, spring cutting of above ground tissues, and summer cutting of aboveground tissues). By performing weekly...
Categories: Data;
Tags: Ann Arbor East,
Aquatic Biology,
Ecology,
Michigan,
USGS Science Data Catalog (SDC),
To determine the differences in soil microbial community composition between native and non-native lineages of Phragmites, we sampled soils from eight sites in the Great Lakes basin where populations of native and non-native Phragmites co-occurred. In addition, we included samples of soils from 27 populations of Phragmites across the Gulf of Mexico and Atlantic Coasts of the US. Samples were collected between July 2015 and September 2017. At each site in the Great Lakes, we sampled rhizosphere and bulk soil surrounding one ramet of each lineage. Samples from Atlantic and Gulf coasts were collected by homogenizing rhizosphere soils from multiple ramets of one population within a single lineage. DNA was extracted...
These data represent the first reference genome for the invasive Phragmites australis ssp. australis (1.14 giga base pairs (Gbp)), as well as output from comparative genomic and transcriptomic analyses for invasive and native genotypes coexisting in the Great Lakes region of North America. Genome sequencing data used tillers and associated rhizome tissues collected from a single P. australis patch at the Ottawa National Wildlife Refuge near Toledo, Ohio, USA. Transcriptome analyses were produced from samples collected from three invasive and three native genotype P. australis patches from four sites around the Great Lakes in Michigan and Ohio, USA.
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