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Description of Work USEPA Region 5 previously funded a study in 2011 evaluating the toxicity of nitrogen compounds to a select number of pond-dwelling amphibians. The objective of this new study will be to determine the toxicity of nitrogen compounds to stream-dwelling amphibians. Nitrogen contamination in aquatic systems results from the decomposition of organic matter, as well as through agricultural inputs, sewage effluents and industrial activities. Nitrogen in its various forms is of ecological concern because of its ubiquitous distribution and toxicity to aquatic life. Nitrogen compounds pose a hazard to aquatic larval stages of amphibians, affecting survival and development, and inducing deformities and behavioral...
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Description of Work The sensitivity of listed species of fish or amphibians was evaluated in acute and chronic toxicity tests conducted with a range of inorganic or organic chemicals with different toxic modes of action. Results of these studies indicate that the acute or chronic sensitivity of rainbow trout, but not fathead minnow, frequently provided protective acute or chronic toxicity thresholds for most of the listed species evaluated. However, only a limited number of species of sculpins or darters were included in this evaluation, and not all species tested occurred in the Great Lakes watershed. The objective of the proposed study will be to: (1) provide additional chronic toxicity data for sculpins or darters...
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Description of Work Currently, a standard test method does not exist for evaluating the effects of sediment-associated toxicants on freshwater mussels. A standard guide for conducting water-only laboratory toxicity tests with freshwater mussels was approved in 2006 (ASTM 2008a) and provides a tested and validated, consensus-based methodology for conducting acute or chronic water toxicity tests with the early life stages (glochidia and juveniles) of freshwater mussels. Several recent peer-reviewed scientific publications have presented data on various toxicants (Bringolf et al. 2007a,b,c; Wang et al. 2007a,b,c) generated with this method and demonstrate consistency and reliability in inter-laboratory (round robin)...
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Description of Work Determine the relative sensitivity of two species of amphibians (i.e. Wood Frogs Rana sylvatica, Cricket Frogs Acris crepitans blanchardi, American Toad Bufo americana) to exposure to acute and chronic levels of nitrate, nitrite and ammonia. This data will be used to evaluate the current ammonia criteria and for possible inclusion in the development of criteria for nitrite and nitrate.
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Description of Work The work completed for this template will provide the following information: 1) Generate acute and chronic toxicity data for freshwater mussels and snails to enhance the protection of listed/endangered species in the GL basin; 2) Refine methods and conduct acute and chronic toxicity tests on amphibians; 3) Develop and test methods for conducting chronic toxicity tests for mayflies.
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Description of Work The sensitivity of various species of freshwater mussels (Unioidea) to range of inorganic and organic chemicals has been evaluated in acute toxicity tests and the sensitivity of freshwater mussels to a range of inorganic chemicals has been evaluated in chronic toxicity tests. However, the chronic toxicity of organic chemicals has only been evaluated with a limited number of mussel species or for a limited number of organic chemicals. The objective of this study will be to evaluate methods for conducting 90-d water only exposures with juvenile mussels and conducted chronic 28-d toxicity tests with at least organic chemicals of concern and two commonly tested species of mussels (Lampsilis siliquoidea,...
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Description of Work Goal of this task is to conduct studies to determine the acute and chronic toxicity of sulfate (as sodium sulfate) to early life stages of: (1) fish (fathead minnow, Pimephales promelas), (2) snails (pond snail Lymnaea stagnalis), and (3) mussels (fatmucket, Lampsilis siliquoidea).
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Description of Work A number of field studies have shown that mayflies (Ephemeroptera) tend to be more sensitive than other benthic macroinvertebrate taxa to elevated levels of total dissolved solids in streams impacted by mining. Until relatively recently, difficulties with culturing have precluded the use of mayflies as laboratory toxicity testing organisms; however, researchers at Stroud Water Research Center have found parthenogenic species that readily reproduce under laboratory conditions, and researchers at the US EPA EERD laboratory have developed laboratory cultured diets for mayflies that will help to move toward standardization of methods. Recent efforts at the Illinois Natural History Survey built upon...
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Description of Work Elevated concentrations of major ions are associated with a variety of discharges to aquatic ecosystems, including urban wastewater, mountain top removal and valley fill operations, and coal bed methane production. There is considerable uncertainty regarding the toxicity of major ions to aquatic organisms, particularly given the variability of constituents that comprise these effluents. Because the relative toxicity of major ions is highly variable, predicting effects based on conductivity, total dissolved solids (TDS) or other composite measures may be simplistic. Furthermore, most of the available information on sensitivity of aquatic organisms to major ions has been derived from short-term...
    map background search result map search result map Toxicity Testing of Mayflies Generation of Chronic Toxicity Data for Sulfate Development and Demonstration of a Sediment Toxicity Test Method with Freshwater Mussels for Assessing Sediment Contaminants in the Great Lakes Basin and within North America Microcosms to Evaluate Elevated Major Ions Toxicity Testing of Sculpins and Darters Toxicity of Organic Chemicals in Mussels Toxicity in Stream-Dwelling Amphibians Determination of the Sensitivity of Two Species of Amphibians to Toxicity From Nitrate, Nitrite and Ammonia Toxicity Testing for Stream Dwelling Amphibians, Mussels, and Mayflies Toxicity Testing for Stream Dwelling Amphibians, Mussels, and Mayflies Determination of the Sensitivity of Two Species of Amphibians to Toxicity From Nitrate, Nitrite and Ammonia Toxicity Testing of Mayflies Generation of Chronic Toxicity Data for Sulfate Development and Demonstration of a Sediment Toxicity Test Method with Freshwater Mussels for Assessing Sediment Contaminants in the Great Lakes Basin and within North America Microcosms to Evaluate Elevated Major Ions Toxicity Testing of Sculpins and Darters Toxicity of Organic Chemicals in Mussels Toxicity in Stream-Dwelling Amphibians