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Location Folder Show Tree ROOT _ScienceBase Catalog __Chesapeake Bay Endocrine Disrupting Chemicals Filters Date Range Past year (1) Past month (0) Past week (0) Past day (0) Custom range... To   Search Extensions ArcGIS Service Definition (1) Types OGC WFS Layer (2) OGC WMS Layer (2) OGC WMS Service (2) ArcGIS REST Map Service (1) ArcGIS Service Definition (1) Downloadable (1) Contacts Jennifer C Brennan Chintamani Kandel U.S. Geological Survey - ScienceBase New York Water Science Center Virginia and West Virginia Water Science Center More... Categories Data (2) Data Release - Revised (1) Tag Types Theme (3) USGS Scientific Topic Keyword (2) Place (3) Harvest Set (3) Tag Schemes None (2) Common geographic areas (2) USGS Thesaurus (2) Geographic Names Information System (GNIS) (1) Marine Realms Information Bank (MRIB) keywords (1) View Results as: JSON ATOM CSV Browse Folder

Development of a dual luciferase-fluorescamine assay adapted to a 384 micro-well plate format-Data We have adapted two commonly used human breast luciferase transactivation cell bioassays, the recently re-named estrogen agonist/antagonist screening VM7Luc4E2 cell bioassay (previously designated BG1Luc4E2) and the androgen/glucocorticoid screening MDA-kb2 cell bioassay, to 384-well formats for HTS of endocrine-active substances. This cost-saving adaptation includes a fast, accurate, and easy measurement of protein amount in each well via the fluorescamine assay (in the form of relative fluorescence units, RFU which are then converted to microgram protein using bovine serum albumin standards) with which to normalize luciferase activity (in the form of relative light units, RLU) of cell lysates without requiring... Categories: Data; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Columbia Environmental Research Center, Missouri, USGS Science Data Catalog (SDC), bioassay, cells, All tags... fluorescence, luciferase, Fewer tags Hormone, pesticide, pharmaceutical and other organic compound data for select water and bed sediment samples collected in Chesapeake Bay watershed in parts of Maryland, Pennsylvania, Virginia, and West Virginia, 2006-2014 These data represent water and bed sediment samples analyzed for a variety of organic compounds. The samples were collected in streams and rivers in the Chesapeake Bay watershed from 2006-2014. Water samples were collected from 61 sites and analyzed for hormones (SH2434 method; Tables 1A and 1B), pharmaceuticals (SH2080 method; Tables 2A and 2B), wastewater indicators (SH1433 method; Tables 3A and 3B), and antibiotics (LCAB method; Tables 4A and 4B). Select water samples were analyzed at the U.S. Geological Survey National Water Quality Laboratory for pesticides (SH2001 method and SH2003 method; Tables 5A/5B and 6A/6B, respectively), wastewater indicators (SH4433 method; Tables 7A and 7B), pharmaceuticals (SH8244... Categories: Data; Types: ArcGIS REST Map Service, ArcGIS Service Definition, Downloadable, Map Service; Tags: Environmental Health, Susquehanna River, Chesapeake Bay, USGS Science Data Catalog (SDC), Water quality Shenandoah River Accumulated Wastewater Ratio Incedental wastewater reuse from streams that receive discharges from Wastewater Treatment Facilities (WWTF) has the potential to be a significant contributor of Endocrine Disrupting Chemicals. An ArcGIS model of WWTFs, NHDPlus Version 2 stream networks (USGS and EPA 2012), and gage stations across the Shenandoah River watershed was created to calculate accumulated wastewater in percent of streamflow (ACCWW%) and Predicted Environmental Concentrations (PECs) of select constituents. Virginia and West Virginia Pollutant Discharge Elimination System (VPDES, WVPDES) discharge facilities, outfall locations, and stream gages were spatially joined to the nearest river segment. Wastewater inputs from outfall locations were... Categories: Data Release - Revised; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Aquatic Biology, Ecology, Environmental Health, Shenandoah River, USGS Science Data Catalog (SDC), All tags... Virginia, Water Quality, Water Resources, inland waterways, wastewater discharge, water, wastewater, hydrology, streamflow, Fewer tags