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Folders: ROOT > ScienceBase Catalog > Chesapeake Bay Endocrine Disrupting Chemicals ( Show all descendants )

Location Folder Show Tree ROOT _ScienceBase Catalog __Chesapeake Bay Endocrine Disrupting Chemicals Filters Date Range Past year (0) Past month (0) Past week (0) Past day (0) Custom range... To   Search Types Map Service (1) OGC WFS Layer (1) OGC WMS Layer (1) OGC WMS Service (1) Contacts Vicki S Blazer Jennifer C Brennan U.S. Geological Survey - ScienceBase Ryan P Braham Eastern Ecological Science Center More... Categories Data (2) Tag Types Theme (2) Place (2) Harvest Set (2) Tag Schemes None (1) USGS Thesaurus (1) Geographic Names Information System (GNIS) (1) Common geographic areas (1) View Results as: JSON ATOM CSV Browse Folder

Development of a dual luciferase-fluorescamine assay adapted to a 384 micro-well plate format-Data We have adapted two commonly used human breast luciferase transactivation cell bioassays, the recently re-named estrogen agonist/antagonist screening VM7Luc4E2 cell bioassay (previously designated BG1Luc4E2) and the androgen/glucocorticoid screening MDA-kb2 cell bioassay, to 384-well formats for HTS of endocrine-active substances. This cost-saving adaptation includes a fast, accurate, and easy measurement of protein amount in each well via the fluorescamine assay (in the form of relative fluorescence units, RFU which are then converted to microgram protein using bovine serum albumin standards) with which to normalize luciferase activity (in the form of relative light units, RLU) of cell lysates without requiring... Categories: Data; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Columbia Environmental Research Center, Missouri, USGS Science Data Catalog (SDC), bioassay, cells, All tags... fluorescence, luciferase, Fewer tags 2004-2010 Chesapeake Bay Smallmouth and Largemouth Bass Estrogenic Biomarker Data The data was gathered to document the extent and severity of biomarkers of exposure to estrogenic chemicals in smallmouth and largemouth bass. Samples were collected from 2004 through 2010 at sites in five watersheds within the Chesapeake Bay watershed. A total of 281 largemouth bass were collected from the Potomac, Susquehanna, Patuxent and Rappahannock rivers and 1,384 smallmouth bass from the Potomac, Susquehanna and James. Fish were collected by boat electroshocking, euthanized in the field, weighed, measured, a blood samples collected and a necropsy completed that included documenting any visible abnormalities and collecting tissues for histopathology. Plasma obtained from blood samples was analyzed for vitellogenin,... Categories: Data; Tags: Maryland, Pennsylvania, USGS Science Data Catalog (SDC), Virginia, West Virginia, All tags... histology, intersex, largemouth bass, smallmouth bass, vitellogenin, Fewer tags