Folder
ROOT
_ScienceBase Catalog
__US Fish and Wildlife Service (USFWS)
___Archive
____Pacific Region, Region 1
_____Pacific Lamprey Data Clearinghouse
______Genetics
_______eDNA
The Pacific lamprey (Entosphenus tridentatus) is an anadromous fish once abundant throughout coastal basins of western North America that has suffered dramatic declines in the last century due primarily to human activities. Here, we describe the development of an environmental DNA (eDNA) assay to detect Pacific lamprey in the Columbia River basin. The eDNA assay successfully amplified tissue derived DNA of Pacific lamprey collected from 12 locations throughout the Columbia River basin. The assay amplifies DNA from other Entosphenus species found outside of the Columbia River basin, but is species-specific within this basin. As a result, the assay presented here may be useful for detecting Entosphenus spp. in geographic...
This pilot study presents an environmental DNA (eDNA) assay for sea lamprey Petromyzon marinus and brown trout Salmo trutta, two species of economic and conservation importance in the Republic of Ireland. The results demonstrate the effectiveness of eDNA for assessing presence of low-abundance taxa (here, P. marinus) for environmental managers, and they highlight the potential for assessing relative abundance of rare or invasive freshwater species.
Lampreys have a worldwide distribution, are functionally important to ecological communities and serve significant roles in many cultures. In Pacific coast drainages of North America, lamprey populations have suffered large declines. However, lamprey population status and trends within many areas of this region are unknown and such information is needed for advancing conservation goals. We developed two quantitative PCR-based, aquatic environmental DNA (eDNA) assays for detection of Pacific Lamprey (Entosphenus tridentatus) and Lampetra spp, using locked nucleic acids (LNAs) in the probe design. We used these assays to characterize the spatial distribution of lamprey in 18 watersheds of Puget Sound, Washington,...
The eDNAtlas is an open-access database developed through crowd-sourced field surveys that provides precise spatial information on the occurrence locations of aquatic species in the U.S. The eDNA samples constituting the database are collected using a standardized field sampling protocol by numerous natural resource agencies and non-governmental organizations partnered with the National Genomics Center for Wildlife and Fish Conservation (NGC), which is a science collaborative within the Rocky Mountain Research Station of the U.S. Forest Service. The eDNAtlas database contains results from thousands of sites and dozens of species and is annually updated with additional results for a growing list of species. Funding...
Invasive sea lamprey (Petromyzon marinus Linnaeus, 1758) are currently managed by the Great Lakes Fishery Commission in an effort to reduce pest populations below levels that cause ecological damage. One technique to improve stream population assessments could be molecular surveillance in the form of environmental DNA (eDNA) monitoring. We developed and validated four probe-based quantitative polymerase chain reaction (qPCR) assays, then used two probes (cytb, nd1) to determine whether eDNA concentration was correlated with adult and larval sea lamprey density in the lab. We found a strong positive correlation between adult sea lamprey densities of 2, 20, and 200 individuals/2000L and eDNA concentrations in tanks...
Control of sea lampreys in the Great Lakes requires accurate assessment of the distribution of this species in natal tributaries and the ability to distinguish sea lampreys from the four lamprey species that are native to the Great Lakes; two of these native species are of conservation concern. Environmental DNA (eDNA) has been used to assess the diversity and abundance of aquatic species by detecting the DNA of an organism in the water instead of locating the organism itself. We developed PCR-based assays to distinguish among the four ‘species’ of Great Lakes lampreys (sea, American brook, chestnut, and silver/northern brook lampreys), and employed these in the development of efficient and cost-effective environmental...
Although Pacific Lampreys were historically abundant in the Pacific Northwest, runs have declined dramatically as a result of barriers to upstream passage, juvenile entrainment, habitat loss, and compromised water quality. Defining the current distribution of Pacific Lamprey is a major component of the Pacific Lamprey Conservation Initiative and is central to lamprey recovery efforts. Environmental DNA (eDNA) sampling may be a time-saving and cost-effective method to broadly assess habitat for Pacific Lamprey presence. However, aquatic macrofaunal eDNA methods are relatively new, with many studies occurring in small streams, and focusing on free-swimming fishes. This pilot study expanded the application of eDNA...
eBLIMP has developed an eDNA marker for Pacific Lamprey and a preliminary set of rangewide occurrence probability maps to assist with future surveys. The maps were developed by combining historical species occurrence observations from the Pacific Lamprey Conservation Initiative.
