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Folders: ROOT > ScienceBase Catalog > USGS Data Release Products > Dissolved organic carbon, total petroleum hydrocarbons and and toxicity assay results for Bemidji, MN (2018) (ver. 2.0, June 2023) ( Show all descendants )

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___Dissolved organic carbon, total petroleum hydrocarbons and and toxicity assay results for Bemidji, MN (2018) (ver. 2.0, June 2023)
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This data describes the relative luminescence units (RLUs) and relative fluorescence units (RFUs) of lysates of aryl-hydrocarbon responsive chemically-activated luciferase expression human hepatoma HG2L7.5c1 cells which were exposed for 4 or 24 h to beta-naphthoflavone dilutions, 2,3,7,8-tetrachlorodibenzo-p-dioxin dilutions, or dilutions of groundwater extracts collected from wells within or near an oil-contaminated area. This data also describes RFUs of bovine serum albumin standards which were used to convert the RFUs of the cell lysates to protein with which to normalize the RLUs of the cell lysates.
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Groundwater samples were collected in June 2018 from a background (reference) well located 200 m upgradient from the source and five wells along a flowline in the plume at 39, 68, 102, 125, and 254 m downgradient from the source. Before sampling, at least three times the water volume in the well casing was purged and field parameters (temperature, dissolved oxygen, specific conductance, and pH) were stable. Two samples from each well were collected into unpreserved 1 L amber bottles and shipped on ice overnight to a commercial lab. The two samples were extracted using dichloromethane (DCM; EPA Method 3510). One sample extract was treated with silica gel cleanup (SGC) column (USEPA method 3630C). Aliquots of the...
    map background search result map search result map Toxicity Assay Data for Groundwater Contaminated by Petroleum Hydrocarbons near Bemidji, MN (2018) CALUX AhR human cell bioassays treated with water extracts from wells near Bemidji, MN USA (2018) Toxicity Assay Data for Groundwater Contaminated by Petroleum Hydrocarbons near Bemidji, MN (2018) CALUX AhR human cell bioassays treated with water extracts from wells near Bemidji, MN USA (2018)