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Mono Lake is a hypersaline soda lake rich in dissolved inorganic arsenic with its primary production currently dominated by Picocystis str. ML. We set out to determine if this picoplankter could metabolize inorganic arsenic, and in doing so form unusual arsenolipids (e.g., methylated arsinoyl ribosides) as reported in other saline ecosystems and by halophilic algae. We cultivated Picocystis str. ML on an inorganic seawater-based medium with either low (37 µM) or high (1,000 µM) phosphate in the presence of arsenite (~0.4 mM), arsenate (~0.8 mM), or without arsenic additions.
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Plant roots have the best-understood mutualisms with microbes, but leaf and bract cell endosymbiosis have not been previously reported. Leaf and bract cells of more than 30 species in 18 families of seed plants were surveyed for the presence of intracellular bacteria and several experiments were designed to find and analyze nutrient exchanges between bacteria and plant cells. This dataset contains the results of 1) histochemical analyses to detect hormones, superoxide, and nitrogenous chemicals around bacteria within plant leaf and bract cells, 2) experiments to assess the differential absorption of isotopic nitrogen into plants, and 3) genetic analysis of bacteria isolated from plant material.


    map background search result map search result map Growth of cultured Picocystis strain ML in the presence of arsenic, and occurrence of arsenolipids in these Picocystis as well as biota and sediment from Mono Lake, California Histochemical study of nitrogen-transfer endosymbiosis Microchemistry demographics and development data from wild caught black carp in the Mississippi River basin, 2011-18 Growth of cultured Picocystis strain ML in the presence of arsenic, and occurrence of arsenolipids in these Picocystis as well as biota and sediment from Mono Lake, California Microchemistry demographics and development data from wild caught black carp in the Mississippi River basin, 2011-18 Histochemical study of nitrogen-transfer endosymbiosis