Elizabeth A Falendysz

Luminescence of AG129 mice infected with recombinant Monkeypox virus expressing firefly luciferase

Adult AG129 mice (129/Sv background deficient in alpha/beta interferon (IFN-α/β) and IFN-Ɣ receptors) were infected with recombinant Monkeypox virus (MPXV)expressing firefly luciferase by either intranasal (IN) or intraperitoneal (IP) routes. Experimental infections were conducted in a BSL-3 laboratory at the USGS National Wildlife Health Center, with a clade II MPXV that expresses firefly luciferase, as described in Osorio et. al, 2009 (MPXV/USA/luc). Twelve AG129 mice (8 males and 4 females) were infected intranasally with 105 plaque forming units (PFU) (5 ul, each nostril) of MPXV/USA/luc. Three control mice were inoculated in the same manner with an equal volume of PBS. Bioluminescent imaging was performed using...

Categories: Data; Tags: Experimental Infection Trial, In vivo imaging, Orthopoxvirus, USGS National Wildlife Health Center; Madison, WI, USGS Science Data Catalog (SDC), All tags... Wildlife Disease, biota, wildlife disease, zoonotic diseases, Fewer tags

Experimental infection of Mexican free-tailed bats (Tadarida brasiliensis) with SARS-CoV-2

We experimentally challenged wild Mexican free-tailed bats (TABR) with SARS-CoV-2 to determine the susceptibility, reservoir potential, and population impacts of infection in this species. Of nine bats oronasally inoculated with SARS-CoV-2, five became infected and orally excreted moderate amounts of virus for up to 18 days post inoculation. These five subjects all seroconverted and cleared the virus before the end of the study with no obvious clinical signs of disease. We additionally found no evidence of viral transmission to uninoculated subjects. These results indicate that while TABR are susceptible to SARS-CoV-2 infection, infection of wild populations of TABR would not likely cause mortality.

Categories: Data; Tags: Mexican free-tailed bats, SARS-CoV-2, USGS National Wildlife Health Center Madison, WI, USGS Science Data Catalog (SDC), Wildlife Disease, All tags... biota, Fewer tags

Viral loads, histology, and adverse events in transgenic mice after passive transfer of serum from black-footed ferrets (Mustela nigripes) used to assess the anti-viral efficacy of a subunit SARS-CoV-2 vaccine candidate

This data set provides biological information from of a captive study of transgenic mice that received either passive serum transfers from black-footed ferrets (BFFs) that were vaccinated against SARS-CoV-2 or BFFs that were unvaccinated, or a monoclonal antibody against SARS-CoV-2 or lastly a placebo. After these treatments mice were challenged with SARS-CoV-2. The data included here have viral loads in the lungs of treated mice, as well as histological results from various tissues collected at the end of the study.

Categories: Data; Tags: Passive serum transfer, SARS-CoV-2, USGS National Wildlife Health Center, Madison, WI, USGS Science Data Catalog (SDC), Wildlife Disease, All tags... biota, black-footed ferrets, experimental challenge, immunogenicity and susceptibility, mustelids, pathology, vaccination, viral loads, Fewer tags

Little Brown Bats (Myotis lucifugus) weights and Ct values for SARS-CoV-2 Infection challenge

Little brown bat (Myotis lucifugus) weights and SARS-CoV2 test results were collected as part of study to assess transmission potential of SARS-CoV2 in North American bat populations. It has been proposed that the SARS-CoV-2 virus originated in Asian bats and subsequently spread through human populations as a pandemic. There is concern that infected humans could transmit the virus to native North American bats, therefore the susceptibility of several North American bat species to the pandemic virus has been experimentally assessed. Big brown bats (Eptesicus fuscus) were shown to be resistant to infection by SARS-CoV-2, while Mexican free-tailed bats (Tadarida brasiliensis) became infected and orally excreted moderate...

Categories: Data; Tags: Myotis lucifugus, National Wildlife Health Center (NWHC), Madison, Wi, SARS-CoV-2, USGS Science Data Catalog (SDC), Wildlife Disease, All tags... biota, challenge, histology, infection, little brown bat, pathology, polymerase chain reaction, resistance, susceptibility, wildlife disease, zoonotic diseases, Fewer tags