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In crude-oil-contaminant plumes the dissolved organic carbon (DOC) is mainly hydrocarbon degradation intermediates only partly quantified by the diesel range total petroleum hydrocarbon (TPHd) method. To understand potential biological effects of degradation intermediates we tested three fractions of DOC: (1) solid phase extract (HLB); (2) dichloromethane (DCM-total) extract used in TPHd; and (3) DCM extract with hydrocarbons isolated by silica gel cleanup (DCM-SGC). Bioactivity of extracts from five wells spanning a range of DOC was tested using an in vitro multiplex reporter system that evaluates modulation of activity of 46 transcription factors; extracts were evaluated at concentrations equivalent to the well...
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This data describes the relative luminescence units and relative fluorescence units of lysates of two estrogen responsive chemically-activated luciferase expression human breast VM7Luc4E2 and VM7LucERbc9 cells which were exposed for 24 hours to estrone and 17beta-estradiol dilutions alone and in mixture with atrazine or water extracts collected from dilutor tanks during a fish exposure study utilizing estrone and atrazine. The data for corresponding measurements of estrone in the water extracts by liquid chromatography-mass spectrometry are also shown. This data also describes relative fluorescence units of bovine serum albumin standards which were used to convert the relative fluorescence units of the cell lysates...
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We have adapted two commonly used human breast luciferase transactivation cell bioassays, the recently re-named estrogen agonist/antagonist screening VM7Luc4E2 cell bioassay (previously designated BG1Luc4E2) and the androgen/glucocorticoid screening MDA-kb2 cell bioassay, to 384-well formats for HTS of endocrine-active substances. This cost-saving adaptation includes a fast, accurate, and easy measurement of protein amount in each well via the fluorescamine assay (in the form of relative fluorescence units, RFU which are then converted to microgram protein using bovine serum albumin standards) with which to normalize luciferase activity (in the form of relative light units, RLU) of cell lysates without requiring...
Categories: Data;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Columbia Environmental Research Center,
Missouri,
USGS Science Data Catalog (SDC),
bioassay,
cells, All tags...
fluorescence,
luciferase, Fewer tags
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Groundwater samples were collected in June 2018 from a background (reference) well located 200 m upgradient from the source and five wells along a flowline in the plume at 39, 68, 102, 125, and 254 m downgradient from the source. Before sampling, at least three times the water volume in the well casing was purged and field parameters (temperature, dissolved oxygen, specific conductance, and pH) were stable. Two samples from each well were collected into unpreserved 1 L amber bottles and shipped on ice overnight to a commercial lab. The two samples were extracted using dichloromethane (DCM; EPA Method 3510). One sample extract was treated with silica gel cleanup (SGC) column (USEPA method 3630C). Aliquots of the...
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This data describes the relative luminescence units (RLUs) and relative fluorescence units (RFUs) of lysates of aryl-hydrocarbon responsive chemically-activated luciferase expression human hepatoma HG2L7.5c1 cells which were exposed for 4 or 24 h to beta-naphthoflavone dilutions, 2,3,7,8-tetrachlorodibenzo-p-dioxin dilutions, or dilutions of groundwater extracts collected from wells within or near an oil-contaminated area. This data also describes RFUs of bovine serum albumin standards which were used to convert the RFUs of the cell lysates to protein with which to normalize the RLUs of the cell lysates.
Categories: Data;
Tags: Bemidji,
CALUX,
PAH,
PAH metabolites,
environment, All tags...
groundwater quality,
oil spills,
oil waste,
pollution,
water pollution, Fewer tags
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