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  4. Quantitation of estrogens in water, alone and in combination with atrazine, using two chemically-activated luciferase expression, CALUX, bioassays and quadrupole-time of flight ultraperformance liquid chromatography/mass spectrometry, UPLC-MS

Quantitation of estrogens in water, alone and in combination with atrazine, using two chemically-activated luciferase expression, CALUX, bioassays and quadrupole-time of flight ultraperformance liquid chromatography/mass spectrometry, UPLC-MS

Dates

Publication Date
Start Date
2016-05-13
End Date
2019-02-11

Citation

Brennan, J.C., Gale, R.W., Henke, A. N., and Tillitt, D.E., 2022, Quantitation of estrogens in water, alone and in combination with atrazine, using two chemically-activated luciferase expression, CALUX, bioassays and quadrupole-time of flight ultraperformance liquid chromatography/mass spectrometry, UPLC-MS: U.S. Geological Survey data release, https://doi.org/10.5066/P98PC526.

Summary

This data describes the relative luminescence units and relative fluorescence units of lysates of two estrogen responsive chemically-activated luciferase expression human breast VM7Luc4E2 and VM7LucERbc9 cells which were exposed for 24 hours to estrone and 17beta-estradiol dilutions alone and in mixture with atrazine or water extracts collected from dilutor tanks during a fish exposure study utilizing estrone and atrazine. The data for corresponding measurements of estrone in the water extracts by liquid chromatography-mass spectrometry are also shown. This data also describes relative fluorescence units of bovine serum albumin standards which were used to convert the relative fluorescence units of the cell lysates to protein with [...]

Contacts

Point of Contact :
Jennifer C Brennan
Process Contact :
Jessica K Leet
Originator :
Jennifer C Brennan, Robert W Gale, Abigail N Henke, Donald E Tillitt
Metadata Contact :
CERC Data Managers
Publisher :
U.S. Geological Survey
Data Owner :
Columbia Environmental Research Center
Distributor :
U.S. Geological Survey - ScienceBase
SDC Data Owner :
Columbia Environmental Research Center
USGS Mission Area :
Ecosystems

Attached Files

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Metadata_Quantitation_of_estrogens_in_water_using_two_CALUX_bioasssays.xml
Original FGDC Metadata
View 		67.67 KB application/fgdc+xml
BSA_CaluxQA.txt 27.7 KB text/plain
CaluxResults_ChemicalStandards.txt 121.54 KB text/plain
CaluxResults_DiluterSamples.txt 749.15 KB text/plain
LCMSResults_DiluterSamples.txt 10.36 KB text/plain

Purpose

The data were collected to obtain estrone concentrations of the dilutor water samples during a laboratory study of largemouth bass and to validate the use of two estrogen responsive chemically-activated luciferase expression human breast VM7Luc4E2 and VM7LucERbc bioassays as a tool for quantitation of estrone concentrations in comparison to liquid chromatography-mass spectrometry.

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Communities

  • Columbia Environmental Research Center (CERC)
  • USGS Data Release Products

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Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P98PC526

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