COMPUTATIONAL BIOLOGIST
Email:
jgreer@usgs.gov
Office Phone:
206-526-2055
Fax:
206-526-6654
ORCID:
0000-0001-6660-9976
Location
6505 N.E. 65th Street
Seattle
, WA
98115
US
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Physiological and molecular endpoints observed in juvenile largemouth bass in response to an estrogen (17α-ethinylestradiol) and subsequently a bacterial challenge (Edwardsiella piscicida) exposure under laboratory conditions. Also included are water quality and chemical concentration data.
Tags: Aquatic Biology,
Columbia Environmental Research Center,
DNA sequencing,
Edwardsiella piscicida,
Environmental Health, All tags...
RNAseq,
USGS Science Data Catalog (SDC),
Western Fisheries Research Science Center laboratory,
alternative complement pathway analysis,
biota,
disease challenge,
estrogenic endocrine disrupting compounds,
histopathology,
immunomodulation,
largemouth bass,
liver,
polymerase chain reaction,
spleen,
survival,
transcriptomic changes, Fewer tags
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Coho salmon have been shown to be highly sensitive to the tire transformation chemical 6PPD-quinone, with concentrations contained in stormwater sufficient to induce mortality in up to 90% of the entire fall coho run. Other salmonid species (chinook, sockeye, chum) are much less sensitive to 6PPD-quinone induced mortality. This data examines the varying species sensitivities across salmoids using in vivo exposures in juvenile fish. We then used established cell lines forom these species to assess their suitability for in vitro studies concerning species differences in toxicokinetics using fluorescence and absorbance-based plate assays.
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From 2018 to 2024 surface waters (fresh and brackish), groundwater, and stormwater (runoff, re-use) samples were collected from sites with varying landuse (agricultural, forested, and urban) and streamflow conditions and analyzed at the Organic Geochemistry Research Laboratory in Lawrence, Kansas for the tire antioxidant 6PPD (6-p-phenylenediamine; N-(1,3-Dimethylbutyl)-N’-phenyl-p-phenylenediamine) and ozonated transformation product 6PPD-quinone (6PPD-Q, 2-((4-Methylpentan-2-yl)amino)-5-(phenylamino)cyclohexa-2,5-diene-1,4-dione)). There were 78 urban sampling sites in Colorado (N=15), Connecticut (N=1), Georgia (N=1), Hawaii (N=22), Kansas (N=7), Michigan (N=1), Minnesota (N=1), North Carolina (N=1), Oklahoma...
Tags: 6PPD,
6PPD-Q,
Aquatic Biology,
Ecology,
Environmental Health, All tags...
Stormwater,
USGS Science Data Catalog (SDC),
United States,
Water Quality,
Water Resources,
environment,
inlandWaters,
liquid chromatography,
mass spectrometry,
stormwater,
tire,
tire and road wear particles,
urban,
water chemistry, Fewer tags
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6PPD-Quinone (6PPD-Q) was recently implicated in inducing mass pre-spawn mortality events in adult coho salmon (Oncorhynchus kisutch) in the Pacific Northwest. Since then, studies have reported highly variable toxicity among fish species, including within the salmonids. This dataset explores the sensitivity of coastal cutthroat trout (Oncorhynchus clarkii clarkii) at three different life stages: alevin, juveniles, and young of the year, and also includes analytically measured concentrations for the alevin and juvenile experiments.
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Coho salmon have been shown to be highly sensitive to the tire-derived chemical 6PPD-quinone. Concentrations contained in stormwater and in urban streams during the fall spawning season are sufficient to induce mortality in up to 90% of the entire fall coho run, threating the long-term survival of populations in urban areas. Another area of concern is survival of coho salmon embryos of adults that successfully reach spawning grounds. This dataset encompasses two separate experiments assessing survival and morphology of embryos exposed to measured concentrations of 6PPD-quinone during development.
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