Research Molecular Biologist
Columbia Environmental Research Center
Email:
crichter@usgs.gov
Office Phone:
573-876-1841
Fax:
573-876-1896
ORCID:
0000-0001-7322-4206
Location
CERC - A-3 Main Office/LAb
4200 New Haven Road
Columbia
, MO
65201
US
Supervisor:
Thea M Edwards
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Description of Work This spring (2014) we will measure Asian carp eDNA over time at a Missouri River site downstream of multiple spawning areas before and during spawning. We will measure the amount of Asian carp eDNA in water samples. The amount of eDNA will be related to the numbers of eggs and larvae counted in matched samples (water samples taken at the same time and place). The time since the eDNA was released by the carp will be estimated and these results will be related to the average age of AC eggs and larvae in matched samples. We will also test for substances which interfere with the eDNA measurement techniques and can lead to false negatives. We will also be validating markers developed by USGS and...
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Interest in the field of environmental DNA (eDNA) is growing rapidly and eDNA surveys are becoming an important consideration for aquatic resource managers dealing with invasive species. However, in order for eDNA monitoring to mature as a research and management tool, there are several critical knowledge gaps that must be filled. One such gap is the fate of eDNA materials in the aquatic environment. Understanding the environmental factors that influence the decay of eDNA and how these factors impact detection probabilities over time and space could have significant implications for eDNA survey design and data interpretation. Here we experimentally explore eDNA decay in waste materials and reproductive cells obtained...
Categories: Data;
Types: Citation;
Tags: Aquatic Biology,
Ecology,
Environmental Health,
Genetics,
Missouri, All tags...
USGS Science Data Catalog (SDC),
eDNA,
environmental factors,
microbial load,
pH,
water temperature, Fewer tags
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Data from metabarcoding assays to detect a suite of mussel species using mitochondrial DNA regions of the cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit (ND1) genes sequences.
Tags: Aquatic Biology,
Clinch River,
Genetics,
USGS Science Data Catalog (SDC),
biodiversity, All tags...
bioinformatic processing,
biota,
degenerate primers,
ecosystem monitoring,
environmental DNA,
freshwater mussels,
high through-put sequencing,
species diversity, Fewer tags
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Data describe a designed environmental DNA (eDNA) survey for the detection of longnose darter (Percina nasuta) in field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater fish species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect the presence of longnose darter DNA. Samples were collected from sites on the St. Francis river, Missouri in 2018.
Tags: Aquatic Biology,
Big creek,
Genetics,
Little St. Francis River,
Marble Creek, All tags...
Missouri,
St. Francis River,
Twelvemile creek,
USGS Science Data Catalog (SDC),
biota,
detection,
eDNA,
freshwater fish,
gear comparison,
longnose darter,
occupancy,
species distribution,
survey methods, Fewer tags
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Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for the spectaclecase (Cumberlandia monodonta), mucket (Actinonaias ligamentina), and fatmucket (Lampsilis siliquoidea) from water samples collected in the laboratory under differing laboratory conditions. Shedding rates were tested under variable mussel biomass, diet, and temperature. Parameters described include descriptions of laboratory collected water samples that were tested to estimate concentration of spectaclecase, mucket or fatmucket DNA qPCR results, and associated quality assurance measurements.
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