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  2. Columbia Environmental Research Center (CERC)
  3. Biochemistry and Physiology Branch
  4. Environmental DNA shedding rates in laboratory conditions for Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea.
  5. Biomass experiments with Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea

Biomass experiments with Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea

Dates

Publication Date
Start Date
2019-10-16
End Date
2021-07-19

Citation

Klymus, K.E., Ruiz-Ramos, D.V., Thompson, N.L., Voshage, M.C. and Richter, C.A., 2023, Environmental DNA shedding rates in laboratory conditions for Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea: U.S. Geological Survey data release, https://doi.org/10.5066/P9IVYWPG.

Summary

Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for the spectaclecase (Cumberlandia monodonta), mucket (Actinonaias ligamentina), and fatmucket (Lampsilis siliquoidea) from water samples collected in the laboratory under differing laboratory conditions. Shedding rates were tested under variable mussel biomass, diet, and temperature. Parameters described include descriptions of laboratory collected water samples that were tested to estimate concentration of spectaclecase, mucket or fatmucket DNA qPCR results, and associated quality assurance measurements.

Contacts

Point of Contact :
Katy E Klymus
Originator :
Katy E Klymus, Dannise V Ruiz Ramos, Nathan L Thompson, Megan C Voshage, Cathy A Richter
Metadata Contact :
CERC Data Managers
Publisher :
U.S. Geological Survey
Data Owner :
Columbia Environmental Research Center
Distributor :
U.S. Geological Survey - ScienceBase
SDC Data Owner :
Columbia Environmental Research Center
USGS Mission Area :
Ecosystems

Attached Files

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ChildMetadata_Biomass_eDNA_Shedding_Rates.xml
Original FGDC Metadata
View 		54.36 KB application/fgdc+xml
Biomass_eDNA_Quality_Assurance.csv 171.05 KB text/csv
Biomass_eDNA_Samples.csv 546.95 KB text/csv
Biomass_IPC_Quality_Assurance.csv 185.92 KB text/csv

Purpose

Environmental DNA data for Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea were collected from laboratory experiments to estimate DNA shedding rates under controlled environmental conditions using variable biomass. These rates will improve our understanding of mussel eDNA detection in the field, ultimately improving our ability to use eDNA for monitoring mussel populations.

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  • Columbia Environmental Research Center (CERC)

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