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We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard samplers, but are more economical and require less maintenance than commercial devices. We conducted wind tunnel comparisons of our two new samplers to Rotorod® samplers using synthetic polyethylene spheres (12 - 160 µm in diameter) to compare numbers and size range of particulates that are captured by the samplers. This dataset contains raw numbers of polyethylene spheres that were captured...
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Rapid ʽŌhiʽa Death (ROD) currently threatens ōhiʽa lehua (Metrosideros polymorpha) on Hawaiʽi Island. First identified in Puna in 2014, the disease has now spread island wide. Besides direct sampling of trees, environmental sampling could serve as an easier and broader strategy to detect Ceratocystis spp., the fungi causing ROD. Environmental sampling could also help monitor the effect of felling ROD infected trees. We developed Passive and Active Environmental Samplers and deployed them at a property in Puna, where both C. lukuohia, and C. huliohia had been detected, and where the land owner practiced the management method of felling infected trees. We set up 2 Active Environmental Samplers (modified mosquito traps...
Categories: Data;
Tags: Ceratocystis,
Environmental DNA,
Environmental Sampler,
Hawaiii,
Management, All tags...
Metrosideros polymorpha,
Ohia lehua,
ROD,
Rapid Ohia Death,
Spore Traps,
environment, Fewer tags
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These data contain the raw quantitative polymerase chain reaction (qPCR) results for all Ceratocystis lukuohia and huliohia testing of environmental DNA (eDNA) collected in the wind column using Passive Environmental Samplers (PES).
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Environmental DNA (eDNA) detection tools are becoming increasingly popular for documenting occurrence and distribution of native and invasive species. These tools can allow early detection of new diseases and invasive species and provide critical information for land management. We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). This dataset contains results of an experiment that was designed to determine probability of detecting known numbers of Ceratocystis lukuohia spores on individual slides in these samplers.
Categories: Data;
Tags: Ceratocystis,
Environmental DNA,
Environmental Sampler,
Hawaiii,
Metrosideros polymorpha, All tags...
Ohia lehua,
ROD,
Rapid Ohia Death,
Spore Traps,
environment, Fewer tags
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These data include metadata and associated data files associated with the manuscript, "Economical Environmental Sampler Designs for Detecting Airborne Spread of Fungi Responsible for Rapid ʽŌhiʽa Death." These data include a total of 8 datasets used for both controlled and field studies evaluating the use of Active (with battery operated fan) and Passive (dependent on wind) USGS Environmental Samplers on Hawaii Island between 2016-2018. Samplers were operated under controlled laboratory and field conditions with a commercial sampler (Rotorod® Model 20) to compare efficacy in capturing synthetic polyethylene spheres (12 - 160 µm in diameter) and also Xyleborus spp. boring dust (frass) known to contain the fungi responsible...
Tags: Ceratocystis,
Ecology,
Environmental DNA,
Environmental Health,
Environmental Sampler, All tags...
Forestry,
Genetics,
Hawaiii,
Metrosideros polymorpha,
Ohia lehua,
Rapid Ohia Death,
Spore Traps,
USGS Science Data Catalog (SDC),
Xyleborus,
environment, Fewer tags
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