Determination of occurrence of hybridization of San Juan River razorback sucker through genetic screening of larval fishes
Citation
Megan V McPhee, Thomas F Turner, Dominique Alo, and W Howard Brandenburg, Determination of occurrence of hybridization of San Juan River razorback sucker through genetic screening of larval fishes: .
Summary
Hybridization is a well-documented threat to the persistence of native suckers throughout the southwestern United States. The objective of this study was to develop DNA-based genetic markers to assess hybrid status of wild-caught razorback sucker (Xyrauchen texanus) larvae in the San Juan River of northwestern New Mexico, southern Colorado and Utah, USA. Wild-caught sucker larvae were screened via polymerase chain reaction (PCR) for genetic variation at two microsatellite DNA loci encoded in the nuclear genome, and a mitochondrial (mt) DNA marker encoded in the control region of the mitochondrion.
Summary
Hybridization is a well-documented threat to the persistence of native suckers throughout the southwestern United States. The objective of this study was to develop DNA-based genetic markers to assess hybrid status of wild-caught razorback sucker (Xyrauchen texanus) larvae in the San Juan River of northwestern New Mexico, southern Colorado and Utah, USA. Wild-caught sucker larvae were screened via polymerase chain reaction (PCR) for genetic variation at two microsatellite DNA loci encoded in the nuclear genome, and a mitochondrial (mt) DNA marker encoded in the control region of the mitochondrion.
