Development, validation, and application of a macroinvertebrate-based Index of Biotic Integrity for nonwadeable rivers of Wisconsin

Quantitative biological assessment protocols are needed for monitoring river status and evaluating river rehabilitation efforts. We conducted a standardized macroinvertebrate survey at 100 sites on 38 nonwadeable rivers in Wisconsin to construct, test, and apply an index of biotic integrity (IBI) intended to be such a bioassessment tool. We assigned independent samples to IBI development (n = 75) and IBI validation (n = 25) data sets. We placed Hester–Dendy artificial substrates at the sites for 6 wk and processed the samples of colonizing macroinvertebrates in the laboratory with a 500-target subsampling procedure plus a large–rare taxon search. Independent of the biota, we assigned an environmental disturbance score to each site [...]