Water erosion results in the mobilization and depletion of soil organic carbon (SOC), but studies providing direct experimental evidence of eroded C mineralization and its linkage to the global C cycle are lacking. A study was conducted to determine the mineralization of SOC in runoff from a southwestern Ohio Crosby soil (fine, mixed, mesic Aeric Ochraqualf) that had been under no-till (NT), chisel till (CT) and moldboard plow (MP) for 38 years. To simulate present and future soil erosion conditions, the 0–3 and 5–8 cm soil layers from triplicate soil blocks extracted from each tillage practice were used. Soil layers were transferred to runoff trays and simulated rainfall (30±5 mm h−1) was applied for 1 h. Runoff was collected at 20, [...]