Conventional and quantitative PCR assays for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples
Dates
Publication Date
2017-08-04
Date Collected
2017-03-05
Date Collected
2017-06-01
Citation
Hutchins, P.R., and Sepulveda, A.J., 2017, Conventional and quantitative PCR assays for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples: U.S. Geological Survey data release, https://doi.org/10.5066/F7C53JDW.
Summary
We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.
Summary
We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.
