The potential implications of climate change to fishes in Great Plains rivers and streams could range from drastic shifts in distribution to extirpation. Many lotic systems in the Great Plains are not well suited for direct escape routes for fish to move to more suitable habitats at other latitudes due to the west-east direction of flows rather than north-south. Therefore, we might expect additional climate related stress on fish communities in the Great Plains compared to other regions of North America. Therefore, we will 1) simulate potential water temperature and flow changes within the Great Plains based on extant regional climate models, 2) assess stream connectivity to potential refugia, 3) develop a database of thermal tolerances by Great Plains stream fishes, and 4) map expected changes in distribution as a result of climate change.