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  4. Enteroctopus Sampling Effects on Genetic Data, Prince William Sound, Alaska, 2012-2015

Enteroctopus Sampling Effects on Genetic Data, Prince William Sound, Alaska, 2012-2015

Dates

Publication Date
Start Date
2012
End Date
2015

Citation

Talbot, S.L., 2017, Enteroctopus sampling effects on genetic data, Prince William Sound, Alaska, 2012-2015: U.S. Geological Survey data release, https://doi.org/10.5066/F7VM49HJ

Summary

These are genetic data collected from replicated samples of 21 Enteroctopus (E. dofleini or a cryptic lineage) in Prince William Sound Alaska to evaluate tissue type, DNA extraction method, and time until analyses are completed on data reliability. Data collected from all samples include two microsatellites identified as possible lineage indicators, and nine microsatellite loci previously identified as polymorphic in both lineages. DNA sequence data from 528 bp of the octopine dehydrogenase (OCDE) gene were also collected.

Contacts

Point of Contact :
U.S. Geological Survey, Alaska Science Center
Originator :
Talbot, Sandra L. (ORCID: 0000-0002-3312-7214)
Metadata Contact :
U.S. Geological Survey, Alaska Science Center
Publisher :
U.S. Geological Survey, Alaska Science Center
Distributor :
U.S. Geological Survey, Alaska Science Center

Attached Files

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octopus_microsat_PWS_talbot_metadata.xml
Original FGDC Metadata
View 		42.85 KB application/fgdc+xml
octopus_microsat_PWS_talbot.zip 25.77 KB application/zip
octopus_microsat_PWS_talbot_metadata.html 89.4 KB text/html

Purpose

Sample source (e.g. blood vs. tissue), storage buffers/techniques, and extraction methods for DNA samples in various species all have been shown to affect DNA quality and quantity. This study of Enteroctopus was undertaken to assess the practicality and reliability of non-invasive sampling techniques (epitehlial swabs) and the use of two microsatellite DNA markers as a lineage indicator to facilitate release of non-target lineages not identifiable by morphology alone.

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  • Alaska Science Center (ASC)

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