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  4. Quantitative polymerase chain reaction (qPCR): An alternative, rapid water quality monitoring tool at a National Park on Lake Michigan.

Quantitative polymerase chain reaction (qPCR): An alternative, rapid water quality monitoring tool at a National Park on Lake Michigan.

Dates

Publication Date
Time Period
2014

Citation

Byappanahalli, M., Shively, D., Spoljaric, A.M., and Otto, C., 2018, Quantitative polymerase chain reaction (qPCR): An alternative, rapid water quality monitoring tool at a National Park on Lake Michigan: U.S. Geological Survey data release, https://doi.org/10.5066/F77P8XND.

Summary

Data were collected to evaluate the efficacy of quantitative polymerase chain reaction (qPCR) as a rapid, alternative method for monitoring recreational water at select beaches and rivers of Sleeping Bear Dunes National Lakeshore (SLBE), in Empire, Michigan. Water samples were collected between August 4 and September 18, 2014 (N=297) from four locations (Esch Rd, Otter Creek, Platte Bay, and Platte River). The samples were analyzed for indicator bacteria, E. coli and enterococci, by both culture-based (membrane filtration, MF; traditional method) and non-culture based (qPCR; rapid method). Recreational water quality standards (RWQS) and beach action values (BAV) were used as indices to compare water quality standard exceedances for [...]

Contacts

Point of Contact :
Muruleedhara Byappanahalli, U.S. Geological, Great Lakes Science Center, Lake Michigan Ecological Research Station
Process Contact :
Christopher Otto, Ashley M Spoljaric
Metadata Contact :
Muruleedhara Byappanahalli
Publisher :
U.S. Geological Survey
Originator :
Muruleedhara Byappanahalli, Dawn A Shively, Ashley M Spoljaric, Christopher Otto
Distributor :
U.S. Geological Survey - ScienceBase
USGS Mission Area :
Ecosystems
SDC Data Owner :
Great Lakes Science Center

Attached Files

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SLBE data release_v4.xml
Original FGDC Metadata
View 		30.03 KB application/fgdc+xml
CultureBasedData.csv 36.47 KB text/csv
Lat_Long.csv 556 Bytes text/csv
qPCR_v2.csv 57.62 KB text/csv

Purpose

These data were collected to evaluate qPCR (DNA based method) as an alternative, rapid water quality-monitoring tool using E. coli and enterococci as fecal indicator bacteria.

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  • Great Lakes Science Center (GLSC)
  • USGS Data Release Products

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Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/F77P8XND

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