Histopathology of zebra finches (Taeniopygia guttata) orally exposed to clothianidin (ver. 2.0, October 2019)
Dates
Publication Date
2018-10-04
Start Date
2018-03-19
End Date
2019-08-12
Revision
2019-10-31
Citation
Lankton, J.S., 2018, Histopathology of zebra finches (Taeniopygia guttata) orally exposed to clothianidin (ver. 2.0, October 2019): U.S. Geological Survey data release, https://doi.org/10.5066/P995179F.
Summary
This dataset reports select histopathological changes in liver, kidney, gonad, spleen, brain, heart, skeletal muscle, lung, or adrenal gland of zebra finches exposed to 15mg/kg, 18mg/kg, 30mg/kg, 60mg/kg, 75mg/kg, 125mg/kg, or 250mg/kg clothianidin (CTD) (with controls) by gavage, in water, or on millet seed either as a single dose or for 7 days. The goal of the project is to develop a subacute neonicotinoid toxicity model using zebra finches as a model passerine. We have completed two acute toxicity trials using CTD insecticide. Based on these studies, we estimate the no observable effect level (NOAEL) and the lowest observable effect level (LOAEL) for CTD in zebra finches to be 15 and 30 mg/kg body weight, respectively, and the concentration [...]
Summary
This dataset reports select histopathological changes in liver, kidney, gonad, spleen, brain, heart, skeletal muscle, lung, or adrenal gland of zebra finches exposed to 15mg/kg, 18mg/kg, 30mg/kg, 60mg/kg, 75mg/kg, 125mg/kg, or 250mg/kg clothianidin (CTD) (with controls) by gavage, in water, or on millet seed either as a single dose or for 7 days. The goal of the project is to develop a subacute neonicotinoid toxicity model using zebra finches as a model passerine. We have completed two acute toxicity trials using CTD insecticide. Based on these studies, we estimate the no observable effect level (NOAEL) and the lowest observable effect level (LOAEL) for CTD in zebra finches to be 15 and 30 mg/kg body weight, respectively, and the concentration producing a toxic effect in 50% of treated birds (EC50) to be approximately 150mg/kg. We next exposed birds for 7 days to CTD in drinking water at nominal doses of 40, 20, 4, and 0mg/kg (cumulative 240, 140, 28, and 0mg/kg CTD). Clinical signs of toxicity were not observed in any bird. Lastly, birds were gavaged daily for 7 days with 18mg/kg body weight CTD dissolved in acetone and mixed with vegetable oil. Clinical signs of toxicity were observed beginning after the fourth exposure day and were similar to signs observed in acute (125 mg/kg) CTD dosing. In order to further refine our model, we re-tested CTD treatment via drinking water (at a higher dose of 60mg/kg), exposed birds to 7 days of CTD coated millet seed (15mg/kg, 30mg/kg and 60mg/kg), and extend the follow-up time following an acute exposure to 21 days. Tissues were scanned on at least 10x objective and lesions potentially associated with neonicotinoid exposure were scored on a severity scale of 0-3 (0: absent; 1: mild; 2: moderate; 3: severe). Despite clinical signs of toxicity, there were no consistent microscopic lesions of exposure at the dose levels examined.
First release: April 10, 2018
Revised: October 31, 2019 (ver. 2.0)
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Lankton ZEFI metadata v2.xml Original FGDC Metadata
View
59.26 KB
application/fgdc+xml
ZEFI version history.txt
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ZEFI adrenal gland lesions v2.csv
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ZEFI brain lesions v2.csv
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ZEFI gonad lesions v2.csv
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ZEFI heart lesions v2.csv
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ZEFI kidney lesions v2.csv
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ZEFI skeletal muscle lesions v2.csv
1.52 KB
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ZEFI lung lesions v2.csv
3.86 KB
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ZEFI liver lesions v2.csv
11.91 KB
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ZEFI spleen lesions v2.csv
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Purpose
The data were collected to help determine whether tissue injury occurred from clothianidin exposure. The results will be included in the publication of the exposure trials.
