Laboratory Incubation results from 2015 for bacterial cell counts, carbon use efficiency, growth efficiency, and dissolved organic matter chemistry from four glacier outflows and four rock glacier outflows in Colorado
Dates
Publication Date
2019-04-10
Citation
Fegel, T., Baron, J.S., Boot, C.M., and Hall, E.K., 2019, Laboratory Incubation results from 2015 for bacterial cell counts, carbon use efficiency, growth efficiency, and dissolved organic matter chemistry from four glacier outflows and four rock glacier outflows in Colorado: U.S. Geological Survey data release, https://doi.org/10.5066/P9A0JNP9.
Summary
Bacterial Data include results of incubations of lakewater bacteria with dissolved organic matter extracted from Colorado glacier or rock glacier outflows in 2015. Cells were counted pre- and post- incubations. The “Counts” tab is the number of cells counted in each view of the microscope using the acridine orange method. “C Calculations” tab is the calculation of carbon as bacterial biomass from cell counts. ”Change in cell counts” is the difference in the number of bacterial cells from the pre-incubation microbial culture and in each assay bottle after the biological oxygen demand incubation. The third tab, “Carbon use efficiency” is calculated as the amount of DOM from oxygen demand assays converted to microbial biomass as carbon. [...]
Summary
Bacterial Data include results of incubations of lakewater bacteria with dissolved organic matter extracted from Colorado glacier or rock glacier outflows in 2015. Cells were counted pre- and post- incubations. The “Counts” tab is the number of cells counted in each view of the microscope using the acridine orange method. “C Calculations” tab is the calculation of carbon as bacterial biomass from cell counts. ”Change in cell counts” is the difference in the number of bacterial cells from the pre-incubation microbial culture and in each assay bottle after the biological oxygen demand incubation. The third tab, “Carbon use efficiency” is calculated as the amount of DOM from oxygen demand assays converted to microbial biomass as carbon. The final two tabs “BGE with calculated RQ” and “BGE with RQ1” are the calculations of bacterial growth efficiency. The first uses a respiratory quotient calculated by using the change in CO2/the change in O2 from the biological oxygen demand assay measurements, and the second is calculated assuming a respiratory quotient of 1, a common practice in freshwater metabolism studies. Dissolved organic matter date is Metabolite Compounds as Identified through Gas Chromatography - Mass Spectrometry (GC-MS) analyses. The “GC-MS Data” tab is the intensities of each compound separated out by individual glacier and rock glacier. The second tab is a table of the top 25 compounds in each glacier and rock glacier, as identified through GC-MS, before and after the biological oxygen demand assays. Included in this table is the fold changes in the mass spectrometry intensities of individual compounds. The third tab is the statistical comparison of each glacier type before and after incubation. “Retention times”, the fourth tab, is the time of detection of each individual compound on the mass spectrometer. Finally, the last tab is a raw data file of all spectra generated through GC-MS including mass spec contaminates, which were removed prior to the metabolite analysis.
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Related External Resources
Type: Related Primary Publication
Fegel, T., Boot, C.M., Broeckling, C.D., Baron, J.S., and Hall, E.K., 2019, Assessing the Chemistry and Bioavailability of Dissolved Organic Matter From Glaciers and Rock Glaciers: Journal of Geophysical Research: Biogeosciences, v. 124, no. 7, p. 1988–2004.
Data were collected to identify the ability of bacteria to metabolize dissolved organic matter from two distinct glacier types, ice and rock glaciers. Rates of bacterial metabolism and dissolved organic matter compounds before and after laboratory incubations are presented. These data could be used to compare how the different contributions of ice glacier and rock glacier dissolved organic matter to high alpine lakes and streams influence ecosystem processes.
